4ZUX
SAGA DUB module Ubp8/Sgf11/Sus1/Sgf73 bound to ubiqitinated nucleosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 178.804, 179.174, 353.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.498 - 3.820 |
R-factor | 0.2369 |
Rwork | 0.234 |
R-free | 0.25590 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.500 |
High resolution limit [Å] | 3.693 |
Rmerge | 0.105 |
Number of reflections | 121413 |
<I/σ(I)> | 7.12 |
Completeness [%] | 99.3 |
Redundancy | 7.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Tris-acetate pH 7.4, 50 mM sodium acetate, 5 mM Mg-acetate, 5% sucrose and 5% 2-propanol |