4XLW

Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Neurogenic locus notch homolog protein 1	polymer	124	13524.2	4	UniProt (Q07008) Pfam (PF07645) Pfam (PF12661) Pfam (PF00008)	Rattus norvegicus (Rat)	Notch 1
2	B, D, F, H (B, D, F, H)	Delta-like protein	polymer	261	29150.7	4	UniProt (D3ZHH1) Pfam (PF07657) Pfam (PF01414) Pfam (PF21795)	Rattus norvegicus (Rat)
3	GA, HA, I, J, K... (G, A, C, E)	CALCIUM ION	non-polymer		40.1	10	Chemie (CA)
4	AA, CA, DA, IA, KA... (E, G, A, C)	beta-D-glucopyranose	non-polymer		180.2	12	Chemie (BGC)
5	BA, JA, M, R (E, G, A, C)	alpha-L-fucopyranose	non-polymer		164.2	4	Chemie (FUC)
6	EA, FA, MA, NA, P... (F, H, B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, C, E, G: 412 - 526 (UniProt: Q07008)

PDB	External Database	Details
Ser 527	-	expression tag
Gly 528	-	expression tag
Arg 529	-	expression tag
Leu 530	-	expression tag
Glu 531	-	expression tag
Val 532	-	expression tag
Leu 533	-	expression tag
Phe 534	-	expression tag
Gln 535	-	expression tag

B, D, F, H: 27 - 283 (UniProt: D3ZHH1)

PDB	External Database	Details
Ser 26	-	expression tag
Ser 28	Gly 28	engineered mutation
Leu 107	Phe 107	engineered mutation
Pro 206	Leu 206	engineered mutation
Lys 257	Asn 257	engineered mutation
Ala 284	-	expression tag
Ala 285	-	expression tag
Ala 286	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	170699.8
Non-Polymers*	Number of molecules	32
	Total formula weight	4546.5
All*	Total formula weight	175246.3

*Water molecules are not included.