4XLW

Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999956
Spacegroup name	P 1 21 1
Unit cell lengths	84.560, 95.570, 122.900
Unit cell angles	90.00, 96.21, 90.00

Refinement procedure

Resolution	84.060 - 3.390
R-factor	0.26
Rwork	0.257
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.719
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (DEV-1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.064	3.600
High resolution limit [Å]	3.390	3.390
Rmerge	0.217	1.220
Number of reflections	27009
<I/σ(I)>	6.12	1.19
Completeness [%]	99.3	96.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	295	PEG 3350, MgSO4, Tris-HCl p, D-(+)-Galactose