4XL1

Complex of Notch1 (EGF11-13) bound to Delta-like 4 (N-EGF1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	Neurogenic locus notch homolog protein 1	polymer	118	12734.3	2	UniProt (Q07008) Pfam (PF07645) Pfam (PF12661) Pfam (PF00008)	Rattus norvegicus (Rat)	Notch 1
2	B, D (B, E)	Delta-like protein	polymer	230	25754.8	2	UniProt (D3ZHH1) Pfam (PF07657) Pfam (PF01414)	Rattus norvegicus (Rat)
3	E, F (C, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	G, H, J, P, Q... (A, D)	beta-D-glucopyranose	non-polymer		180.2	6	Chemie (BGC)
5	I, R (A, D)	alpha-L-fucopyranose	non-polymer		164.2	2	Chemie (FUC)
6	K, L, M, T, U... (A, D)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
7	N, O, W, X (B, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
8	AA, BA, Y, Z (D, E, A, B)	water	water		18.0	325	Chemie (HOH)

Sequence modifications

A, D: 412 - 526 (UniProt: Q07008)

PDB	External Database	Details
Pro 411	-	expression tag
Ser 527	-	expression tag
Gly 528	-	expression tag

B, E: 27 - 252 (UniProt: D3ZHH1)

PDB	External Database	Details
Gly 25	-	expression tag
Ser 26	-	expression tag
Ser 28	Gly 28	engineered mutation
Leu 107	Phe 107	engineered mutation
Pro 206	Leu 206	engineered mutation
Ala 253	-	expression tag
Ala 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76978.2
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	18
	Total formula weight	2534.5
All*	Total formula weight	80361.6

*Water molecules are not included.