4TU7
Structure of U2AF65 D231V variant with BrU5 DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Splicing factor U2AF 65 kDa subunit | polymer | 174 | 19059.8 | 2 | UniProt (P26368) Pfam (PF00076) | Homo sapiens (Human) | U2 auxiliary factor 65 kDa subunit,hU2AF65,U2 snRNP auxiliary factor large subunit |
| 2 | C, D (P, E) | DNA (5'-D(*UP*UP*UP*UP*(BRU)P*UP*U)-3') | polymer | 7 | 2065.1 | 2 | synthetic construct | ||
| 3 | E, F, M, N (A, B) | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 4 | Chemie (DIO) | |||
| 4 | G, H, I, P, Q... (A, B) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 5 | J, K, L, S (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | O (B) | N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE | non-polymer | 862.1 | 1 | Chemie (CPQ) | |||
| 7 | T, U, V, W (A, B, P, E) | water | water | 18.0 | 249 | Chemie (HOH) |
Sequence modifications
A, B: 148 - 336 (UniProt: P26368)
| PDB | External Database | Details |
|---|---|---|
| Gly 143 | - | expression tag |
| Pro 144 | - | expression tag |
| Leu 145 | - | expression tag |
| Gly 146 | - | expression tag |
| Ser 147 | - | expression tag |
| Val 231 | Asp 231 | engineered mutation |
| - | Met 238 | deletion |
| - | Ser 239 | deletion |
| - | Glu 240 | deletion |
| - | Asn 241 | deletion |
| - | Pro 242 | deletion |
| - | Ser 243 | deletion |
| - | Val 244 | deletion |
| - | Tyr 245 | deletion |
| - | Val 246 | deletion |
| - | Pro 247 | deletion |
| - | Gly 248 | deletion |
| - | Val 249 | deletion |
| - | Val 250 | deletion |
| - | Ser 251 | deletion |
| - | Thr 252 | deletion |
| - | Val 253 | deletion |
| - | Val 254 | deletion |
| - | Pro 255 | deletion |
| - | Asp 256 | deletion |
| - | Ser 257 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 42249.8 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 2159.2 | |
| All* | Total formula weight | 44409.0 |
