4TU7

Structure of U2AF65 D231V variant with BrU5 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	164.432, 37.361, 100.192
Unit cell angles	90.00, 125.60, 90.00

Refinement procedure

Resolution	35.982 - 2.087
R-factor	0.1789
Rwork	0.174
R-free	0.22300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.208
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.447	39.447	2.200
High resolution limit [Å]	2.087	6.600	2.087
Rmerge		0.017	0.228
Rmeas	0.044
Rpim	0.025	0.011	0.154
Total number of observations	86388	2825	11901
Number of reflections	28628
<I/σ(I)>	18.9	44.3	4.6
Completeness [%]	95.7	95.1	91
Redundancy	3	2.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.0, DEOXY-BIG CHAP