4RWO

Crystal structure of the porcine OAS1 L149R mutant in complex with dsRNA and ApCpp in the AMP donor position

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	RNA (5'-R(*GP*GP*CP*UP*UP*UP*UP*GP*AP*CP*CP*UP*UP*UP*AP*UP*GP*AP*A)-3')	polymer	19	6017.6	1		SYNTHETIC CONSTRUCT
2	B (C)	RNA (5'-R(*UP*UP*CP*AP*UP*AP*AP*AP*GP*GP*UP*CP*AP*AP*AP*AP*GP*CP*C)-3')	polymer	19	6069.7	1		SYNTHETIC CONSTRUCT
3	C (A)	2'-5'-oligoadenylate synthase 1	polymer	357	41322.4	1	UniProt (Q29599) Pfam (PF01909) Pfam (PF10421)	Sus scrofa (pigs swine wild boar)	(2-5')oligo(A) synthase 1, 2-5A synthase 1, p42 OAS
4	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	F (A)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	1	Chemie (APC)
6	G, H, I (B, C, A)	water	water		18.0	73	Chemie (HOH)

Sequence modifications

A: 1 - 349 (UniProt: Q29599)

PDB	External Database	Details
Arg 149	Leu 149	engineered mutation
Gly 350	-	expression tag
Ser 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	53409.6
Non-Polymers*	Number of molecules	3
	Total formula weight	553.8
All*	Total formula weight	53963.4

*Water molecules are not included.