4RWO
Crystal structure of the porcine OAS1 L149R mutant in complex with dsRNA and ApCpp in the AMP donor position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.06450 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.100, 73.100, 208.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.306 - 2.200 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.306 |
High resolution limit [Å] | 2.200 |
Number of reflections | 29636 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 100 mM Tris-HCl pH 8.0, 25% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K |