4QWT
Anaerobic crystal structure of delta413-417:GS LOX in complex with arachidonate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Allene oxide synthase-lipoxygenase protein | polymer | 696 | 79409.0 | 4 | UniProt (O16025) Pfam (PF01477) Pfam (PF00305) In PDB | Plexaura homomalla (black sea rod) | Arachidonate 8-lipoxygenase |
| 2 | D, A, C, B | FE (II) ION | non-polymer | 55.8 | 4 | Chemie (FE2) | |||
| 3 | D, A, C, B | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 4 | B, C, D, A | ACETATE ION | non-polymer | 59.0 | 25 | Chemie (ACT) | |||
| 5 | C, B, D, A | GLYCEROL | non-polymer | 92.1 | 17 | Chemie (GOL) | |||
| 6 | B, D, A, C | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 7 | C | ARACHIDONIC ACID | non-polymer | 304.5 | 1 | Chemie (ACD) | |||
| 8 | water | water | 18.0 | 1795 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 4 - 693 (UniProt: O16025)
| PDB | External Database | Details |
|---|---|---|
| Met -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| - | Trp 413 | deletion |
| - | Phe 414 | deletion |
| - | His 415 | deletion |
| Gly 43 | Asn 416 | engineered mutation |
| Ser 44 | Asp 417 | engineered mutation |
| Ile 409 | Val 782 | conflict |
| Ile 590 | Val 963 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 317636.0 | |
| Non-Polymers* | Number of molecules | 61 |
| Total formula weight | 4102.9 | |
| All* | Total formula weight | 321738.9 |
