4QWT
Anaerobic crystal structure of delta413-417:GS LOX in complex with arachidonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.030, 170.670, 104.720 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 48.416 - 2.002 |
| R-factor | 0.1622 |
| Rwork | 0.162 |
| R-free | 0.21550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gf1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.813 |
| Number of reflections | 242387 | |
| <I/σ(I)> | 11.68 | 1.9 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 6-8% PEG8000, 5% glycerol, 0.2 M calcium chloride, 0.1 M imidazole acetate, pH 8.0, anaerobic, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
