4KFM

Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with the beta-gamma G protein subunits

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	G protein-activated inward rectifier potassium channel 2	polymer	340	39062.0	1	UniProt (Q8C4T8) Pfam (PF01007) Pfam (PF17655) UniProt (by SIFTS) (P48542) In PDB	Mus musculus (mouse)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7717.9	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	A	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	1	Chemie (PIO)
5	A	POTASSIUM ION	non-polymer		39.1	5	Chemie (K)
6	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	A	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	1	Chemie (LMT)

Sequence modifications

A: 52 - 380 (UniProt: Q8C4T8)

PDB	External Database	Details
Met 50	-	EXPRESSION TAG
Ala 51	-	EXPRESSION TAG
Ser 381	-	EXPRESSION TAG
Asn 382	-	EXPRESSION TAG
Ser 383	-	EXPRESSION TAG
Leu 384	-	EXPRESSION TAG
Glu 385	-	EXPRESSION TAG
Val 386	-	EXPRESSION TAG
Leu 387	-	EXPRESSION TAG
Phe 388	-	EXPRESSION TAG
Gln 389	-	EXPRESSION TAG

G: 1 - 68 (UniProt: P59768)

PDB	External Database	Details
Gly -1	-	EXPRESSION TAG
Pro 0	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	84196.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1475.7
All*	Total formula weight	85672.5

*Water molecules are not included.