4KFM
Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with the beta-gamma G protein subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | MAR |
| Wavelength(s) | 1.034 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 127.308, 127.308, 309.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.890 - 3.450 |
| R-factor | 0.2296 |
| Rwork | 0.228 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.045 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 7.430 | 3.450 |
| Rmerge | 0.226 | 0.093 | |
| Number of reflections | 16537 | ||
| <I/σ(I)> | 3.5 | ||
| Completeness [%] | 96.0 | 99.5 | 72.5 |
| Redundancy | 10.6 | 10 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293.15 | 600 mM NaK tartrate, 50 mM Na-ADA, pH 5.8, vapor diffusion, hanging drop, temperature 293.15K |
