4GEY

High pH structure of Pseudomonas putida OprB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Porin B	polymer	436	49054.4	1	UniProt (E4R6F8) Pfam (PF04966) UniProt (by SIFTS) (A5VZA8) In PDB	Pseudomonas putida
2	A	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
3	A	(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE	non-polymer		306.4	8	Chemie (C8E)
4	A	DECYL-BETA-D-MALTOPYRANOSIDE	non-polymer		482.6	4	Chemie (DMU)
5	A	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6		water	water		18.0	47	Chemie (HOH)

Sequence modifications

A: 1 - 421 (UniProt: E4R6F8)

PDB	External Database	Details
Ala -14	-	expression tag
Asn -13	-	expression tag
Val -12	-	expression tag
Arg -11	-	expression tag
Leu -10	-	expression tag
Gln -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Leu -1	-	expression tag
Glu 0	-	expression tag
Mse 220	Leu 246	engineered mutation
Mse 222	Val 248	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49054.4
Non-Polymers*	Number of molecules	14
	Total formula weight	4656.9
All*	Total formula weight	53711.3

*Water molecules are not included.