4GEY

High pH structure of Pseudomonas putida OprB

Summary for 4GEY

• Entry DOI: 10.2210/pdb4gey/pdb
• Related: 4GF4
• Descriptor: Porin B, beta-D-glucopyranose, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, ... (6 entities in total)
• Functional Keywords: beta-barrel, outer membrane channel, carbohydrate transport, transport protein
• Biological source: Pseudomonas putida
• Total number of polymer chains: 1
• Total formula weight: 53711.30

Authors

van den Berg, B. (deposition date: 2012-08-02, release date: 2012-10-24, Last modification date: 2020-07-29)

Primary citation

van den Berg, B.
Structural basis for outer membrane sugar uptake in pseudomonads.
J.Biol.Chem., 287:41044-41052, 2012

PubMed Abstract: Substrate-specific outer membrane channels of gram-negative bacteria mediate uptake of many small molecules, including carbohydrates. The mechanism of sugar uptake by enterobacterial channels, such as Escherichia coli LamB (maltoporin), has been characterized in great detail. In pseudomonads and related organisms, sugar uptake is not mediated by LamB but by OprB channels. Beyond the notion that OprB channels seem to prefer monosaccharides as substrates, very little is known about OprB-mediated sugar uptake. Here I report the X-ray crystal structure of an OprB channel from Pseudomonas putida F1. The structure shows that OprB forms a monomeric, 16-stranded β-barrel with a constriction formed by extracellular loops L2 and L3. The side chains of two highly conserved arginine residues (Arg(83) and Arg(110)) and a conserved glutamate (Glu(106)) line the channel constriction and interact with a bound glucose molecule. Liposome swelling uptake assays show a strong preference for monosaccharide transport over disaccharides. Moreover, substrates with a net negative charge are disfavored by the channel, probably due to the negatively charged character of the constriction. The architecture of the eyelet and the absence of a greasy slide provide an explanation for the observed specificity of OprB for monosaccharides rather than the oligosaccharides preferred by LamB and related enterobacterial channels.

PubMed: 23066028
DOI: 10.1074/jbc.M112.408518

Experimental method

X-RAY DIFFRACTION (2.7 Å)