4GEY
High pH structure of Pseudomonas putida OprB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-19 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.343, 88.343, 177.706 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.980 - 2.700 |
R-factor | 0.193 |
Rwork | 0.188 |
R-free | 0.23900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 22711 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 293 | 50 mM Tris-HCl, 0.2 M Sodium-phosphate, 10% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |