4GEY
High pH structure of Pseudomonas putida OprB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-19 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.343, 88.343, 177.706 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 2.700 |
| R-factor | 0.193 |
| Rwork | 0.188 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 22711 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 50 mM Tris-HCl, 0.2 M Sodium-phosphate, 10% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
