4FO6

Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a dATP analog opposite a templating T and an rCMP at the primer terminus.

Replaces:  3UQ1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase lambda	polymer	329	36704.0	1	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)	Pol Lambda, DNA polymerase beta-2, Pol beta2, DNA polymerase kappa
2	B (T)	5'-D(*CP*GP*GP*CP*TP*GP*TP*AP*CP*TP*G)-3'	polymer	11	3365.2	1
3	C (P)	5'-D(*CP*AP*GP*TP*AP)-R(P*C)-3'	polymer	6	1809.2	1
4	D (D)	5'-D(P*GP*CP*CP*G)-3'	polymer	4	1191.8	1
5	E (H)	5'-D(P*TP*AP*CP*TP*G)-3'	polymer	5	1495.0	1
6	F (I)	5'-D(P*CP*AP*GP*TP*A)-3'	polymer	5	1504.0	1
7	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	H, K (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
9	I (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
10	J (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
11	L (A)	2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]adenosine	non-polymer		489.2	1	Chemie (F2A)
12	M, N, O, P, Q... (A, T, P, D, H...)	water	water		18.0	212	Chemie (HOH)

Sequence modifications

A: 242 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Lys 463	Ser 463	SEE REMARK 999
Gly 464	Gln 464	SEE REMARK 999
Glu 470	Gln 470	SEE REMARK 999
Thr 471	Gln 471	SEE REMARK 999
Ala 543	Cys 543	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	46069.3
Non-Polymers*	Number of molecules	6
	Total formula weight	649.9
All*	Total formula weight	46719.2

*Water molecules are not included.