4FO6
Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a dATP analog opposite a templating T and an rCMP at the primer terminus.
Replaces: 3UQ1Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase lambda | polymer | 329 | 36704.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB | Homo sapiens (human) | Pol Lambda, DNA polymerase beta-2, Pol beta2, DNA polymerase kappa |
2 | T | 5'-D(*CP*GP*GP*CP*TP*GP*TP*AP*CP*TP*G)-3' | polymer | 11 | 3365.2 | 1 | |||
3 | P | 5'-D(*CP*AP*GP*TP*AP)-R(P*C)-3' | polymer | 6 | 1809.2 | 1 | |||
4 | D | 5'-D(P*GP*CP*CP*G)-3' | polymer | 4 | 1191.8 | 1 | |||
5 | H | 5'-D(P*TP*AP*CP*TP*G)-3' | polymer | 5 | 1495.0 | 1 | |||
6 | I | 5'-D(P*CP*AP*GP*TP*A)-3' | polymer | 5 | 1504.0 | 1 | |||
7 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
8 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
9 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
10 | A | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
11 | A | 2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]adenosine | non-polymer | 489.2 | 1 | Chemie (F2A) | |||
12 | water | water | 18.0 | 212 | Chemie (HOH) |
Sequence modifications
A: 242 - 575 (UniProt: Q9UGP5)
PDB | External Database | Details |
---|---|---|
Lys 463 | Ser 463 | SEE REMARK 999 |
Gly 464 | Gln 464 | SEE REMARK 999 |
Glu 470 | Gln 470 | SEE REMARK 999 |
Thr 471 | Gln 471 | SEE REMARK 999 |
Ala 543 | Cys 543 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 46069.3 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 649.9 | |
All* | Total formula weight | 46719.2 |