4FO6

Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a dATP analog opposite a templating T and an rCMP at the primer terminus.

Replaces:  3UQ1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003887	molecular_function	DNA-directed DNA polymerase activity
A	0006281	biological_process	DNA repair
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034061	molecular_function	DNA polymerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 601

Chain	Residue
A	ASP427
A	ASP429
A	MN604
A	F2A606
A	HOH701

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA A 602

Chain	Residue
A	CYS300
A	ILE302
A	ILE305
D	DC3

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 603

Chain	Residue
A	ARG517
A	GLU529
T	DG6

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MN A 604

Chain	Residue
A	ASP427
A	ASP429
A	ASP490
A	MG601
A	F2A606
A	HOH823
P	C6

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 605

Chain	Residue
A	SER339
A	ILE341
A	ALA344
A	HOH704
A	HOH728
P	DA5

---

site_id	AC6
Number of Residues	25
Details	BINDING SITE FOR RESIDUE F2A A 606

Chain	Residue
A	ARG386
A	GLY416
A	SER417
A	ARG420
A	CYS425
A	GLY426
A	ASP427
A	ASP429
A	TYR505
A	PHE506
A	THR507
A	GLY508
A	ALA510
A	ASN513
A	MG601
A	MN604
A	HOH701
A	HOH703
A	HOH735
A	HOH737
A	HOH782
A	HOH823
P	C6
T	DT5
T	DG6

---

Functional Information from PROSITE/UniProt

site_id	PS00522
Number of Residues	20
Details	DNA_POLYMERASE_X DNA polymerase family X signature. GSYrRGkatCgDVDVLIthP

Chain	Residue	Details
A	GLY416-PRO435

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Schiff-base intermediate with DNA => ECO:0000269|PubMed:11457865

Chain	Residue	Details
A	LYS312

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:17475573, ECO:0007744|PDB:2PFP, ECO:0007744|PDB:2PFQ

Chain	Residue	Details
A	ARG386
A	SER417
A	GLY426
A	ALA518

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:17475573, ECO:0007744|PDB:2PFO, ECO:0007744|PDB:2PFQ

Chain	Residue	Details
A	ASP427
A	ASP429
A	PRO495

---