4FO6
Crystal structure of the pre-catalytic ternary complex of polymerase lambda with a dATP analog opposite a templating T and an rCMP at the primer terminus.
Replaces: 3UQ1Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.780, 63.655, 141.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.027 - 2.007 |
R-factor | 0.1918 |
Rwork | 0.190 |
R-free | 0.23060 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3mgi |
RMSD bond length | 0.011 |
RMSD bond angle | 1.159 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_868)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 32824 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 95.5 | 78.4 |
Redundancy | 4.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50mM MES pH 6.5, 9%(v/v) PEG400, 100mM KCL, 10mM MGCL2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |