4FBK

Crystal structure of a covalently fused Nbs1-Mre11 complex with one manganese ion per active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA repair and telomere maintenance protein nbs1,DNA repair protein rad32 CHIMERIC PROTEIN	polymer	472	53351.9	2	UniProt (O43070) UniProt (Q09683) Pfam (PF00149) Pfam (PF04152)	Schizosaccharomyces pombe
2	C, D, E, G (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F, H (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	I, J (A, B)	water	water		18.0	210	Chemie (HOH)

Sequence modifications

A, B: 474 - 531 (UniProt: O43070)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

A, B: 1015 - 1413 (UniProt: Q09683)

PDB	External Database	Details
Val 532	-	linker
Asp 533	-	linker
Gly 534	-	linker
Ser 535	-	linker
Ala 536	-	linker
Gly 537	-	linker
Ser 538	-	linker
Ala 539	-	linker
Gly 540	-	linker
Ser 541	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	106703.8
Non-Polymers*	Number of molecules	6
	Total formula weight	494.1
All*	Total formula weight	107198.0

*Water molecules are not included.