4FBK
Crystal structure of a covalently fused Nbs1-Mre11 complex with one manganese ion per active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.261, 78.872, 222.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.884 - 2.379 |
R-factor | 0.2232 |
Rwork | 0.222 |
R-free | 0.24170 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.777 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.884 |
High resolution limit [Å] | 2.379 |
Number of reflections | 43368 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 MM Tri Na Citrate pH 5.0, 200 mM ammonium sulfate, 13% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |