4C2B

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with Disulfide Mutation in Complex with High Affinity GPIb alpha

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	VON WILLEBRAND FACTOR	polymer	215	24706.7	4	UniProt (P04275) Pfam (PF00092) In PDB	HOMO SAPIENS (HUMAN)	VWF, VON WILLEBRAND ANTIGEN II, VON WILLEBRAND FACTOR A1
2	B, D, F, H	PLATELET GLYCOPROTEIN IB ALPHA CHAIN	polymer	291	32492.1	4	UniProt (P07359) Pfam (PF01462) Pfam (PF13855) In PDB	HOMO SAPIENS (HUMAN)	GP-IB ALPHA, GPIB-ALPHA, GPIBA, GLYCOPROTEIN IBALPHA, ANTIGEN CD42B-ALPHA
3	A, B, C, D, E...	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
4	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
5	G	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	65	Chemie (HOH)

Sequence modifications

A, C, E, G: 1264 - 1471 (UniProt: P04275)

PDB	External Database	Details
Met 1263	-	expression tag
His 1472	-	expression tag
His 1473	-	expression tag
His 1474	-	expression tag
His 1475	-	expression tag
His 1476	-	expression tag
His 1477	-	expression tag
Cys 1271	Tyr 1271	engineered mutation
Arg 1272	Cys 1272	engineered mutation
Ala 1381	Thr 1381	variant

B, D, F, H: 1 - 290 (UniProt: P07359)

PDB	External Database	Details
Gly 291	-	expression tag
Arg 21	Asn 37	engineered mutation
Arg 159	Asn 175	engineered mutation
Val 233	Gly 249	engineered mutation
Val 239	Met 255	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	228795.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1262.0
All*	Total formula weight	230057.1

*Water molecules are not included.