3ZY7

Crystal structure of computationally redesigned gamma-adaptin appendage domain forming a symmetric homodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	AP-1 COMPLEX SUBUNIT GAMMA-1	polymer	122	13637.4	2	UniProt (P22892) Pfam (PF02883) In PDB	MUS MUSCULUS (HOUSE MOUSE)	REDESIGNED CLATHRIN ADAPTOR AP1, ADAPTER-RELATED PROTEIN CO ADAPTOR PROTEIN COMPLEX AP-1 SUBUNIT GAMMA-1, CLATHRIN ASSEMBLY PROTEIN COMPLEX 1 GAMMA-1 LARGE CHAIN, GAMMA-ADAPTIN, GAMMA1-ADAPTIN, GOLGI ADAPTOR HA1/AP1 ADAPTIN SUBUNIT GAMMA-1
2	A, B	ISOPROPYL ALCOHOL	non-polymer		60.1	2	Chemie (IPA)
3	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4		water	water		18.0	298	Chemie (HOH)

Sequence modifications

A, B: 2 - 120 (UniProt: P22892)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag
Asp 9	Ser 711	engineered mutation
Ala 10	Lys 712	engineered mutation
Leu 11	Asn 713	engineered mutation
Phe 98	Tyr 800	engineered mutation
Trp 100	His 802	engineered mutation
Asn 101	Lys 803	engineered mutation
Tyr 103	Ser 805	engineered mutation
Lys 104	Ala 806	engineered mutation
Val 105	Met 807	engineered mutation
Ser 107	Asp 809	engineered mutation
Glu 108	Leu 810	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27274.9
Non-Polymers*	Number of molecules	4
	Total formula weight	332.4
All*	Total formula weight	27607.3

*Water molecules are not included.