3ZY7
Crystal structure of computationally redesigned gamma-adaptin appendage domain forming a symmetric homodimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | AP-1 COMPLEX SUBUNIT GAMMA-1 | polymer | 122 | 13637.4 | 2 | UniProt (P22892) Pfam (PF02883) | MUS MUSCULUS (HOUSE MOUSE) | REDESIGNED CLATHRIN ADAPTOR AP1, ADAPTER-RELATED PROTEIN CO ADAPTOR PROTEIN COMPLEX AP-1 SUBUNIT GAMMA-1, CLATHRIN ASSEMBLY PROTEIN COMPLEX 1 GAMMA-1 LARGE CHAIN, GAMMA-ADAPTIN, GAMMA1-ADAPTIN, GOLGI ADAPTOR HA1/AP1 ADAPTIN SUBUNIT GAMMA-1 |
| 2 | C, F (A, B) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 3 | D, E (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 298 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 120 (UniProt: P22892)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Met 1 | - | expression tag |
| Asp 9 | Ser 711 | engineered mutation |
| Ala 10 | Lys 712 | engineered mutation |
| Leu 11 | Asn 713 | engineered mutation |
| Phe 98 | Tyr 800 | engineered mutation |
| Trp 100 | His 802 | engineered mutation |
| Asn 101 | Lys 803 | engineered mutation |
| Tyr 103 | Ser 805 | engineered mutation |
| Lys 104 | Ala 806 | engineered mutation |
| Val 105 | Met 807 | engineered mutation |
| Ser 107 | Asp 809 | engineered mutation |
| Glu 108 | Leu 810 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 27274.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 332.4 | |
| All* | Total formula weight | 27607.3 |
