3ZY7
Crystal structure of computationally redesigned gamma-adaptin appendage domain forming a symmetric homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-26 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.636, 44.248, 53.017 |
Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
Resolution | 23.135 - 1.090 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.18060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a7b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.399 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.140 | 1.100 |
High resolution limit [Å] | 1.090 | 1.090 |
Rmerge | 0.050 | 0.330 |
Number of reflections | 90534 | |
<I/σ(I)> | 33.1 | 1.9 |
Completeness [%] | 92.6 | 44.6 |
Redundancy | 3.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | PROTEIN SOLUTION: 20MM MES PH 6M 150MM NACL WELL SOLUTION: 0.1M NA ACETATE PH 5, 20% (W/V) PEG 8K, 6% (V/V) 2-PROPANOL CRYOPROTECTANT: WELL SOLUTION PLUS 15% (V/V) ETHYLENE GLYCOL |