3OLB

Poliovirus polymerase elongation complex with 2',3'-dideoxy-ctp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M	Polymerase	polymer	471	53761.2	4	UniProt (B3VQP5) Pfam (PF00680) In PDB	Human poliovirus 1
2	B, F, J, N	RNA (5'-R(*AP*AP*GP*UP*CP*U*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*UP*CP*CP*GP*GP*AP*AP*A)-3')	polymer	26	8280.0	4
3	C, G, K, O	RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*A)-3')	polymer	14	4563.8	4
4	D, H, L, P	RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	9	2974.9	4
5	I, M, A, E	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	I, M, A, E	2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE	non-polymer		451.2	4	Chemie (DCT)
7	I, M, O, A, E	ISOPROPYL ALCOHOL	non-polymer		60.1	12	Chemie (IPA)
8		water	water		18.0	898	Chemie (HOH)

Sequence modifications

A, E, I, M: 1 - 461 (UniProt: B3VQP5)

PDB	External Database	Details
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	278319.4
Non-Polymers*	Number of molecules	20
	Total formula weight	2787.4
All*	Total formula weight	281106.8

*Water molecules are not included.