3OLB
Poliovirus polymerase elongation complex with 2',3'-dideoxy-ctp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-08 |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 60.528, 60.539, 192.936 |
Unit cell angles | 83.82, 83.84, 77.35 |
Refinement procedure
Resolution | 45.226 - 2.410 |
R-factor | 0.2196 |
Rwork | 0.217 |
R-free | 0.26130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.063 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.230 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.072 | 0.646 |
Number of reflections | 149534 | |
<I/σ(I)> | 4.3 | 1 |
Completeness [%] | 96.8 | 94.5 |
Redundancy | 1.97 | 1.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 95 mM trisodium citrate, pH 5.6-5.9, 6.5-7.0% (v/v) isopropanol, 16.5-18.0% (w/v) PEG4000, 5% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 283K |