3OJ4

Crystal structure of the A20 ZnF4, ubiquitin and UbcH5A complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ubiquitin-conjugating enzyme E2 D1	polymer	153	17277.8	2	UniProt (P51668) Pfam (PF00179)	Homo sapiens (human)	Ubiquitin-protein ligase D1, Ubiquitin carrier protein D1, UbcH5, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-conjugating enzyme E2(17)KB 1, UBC4/5 homolog, Stimulator of Fe transport, SFT
2	B, E (B, E)	Ubiquitin	polymer	79	8859.1	2	UniProt (P0CG47) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
3	C, F (C, F)	Tumor necrosis factor alpha-induced protein 3	polymer	49	5418.1	2	UniProt (P21580)	Homo sapiens (human)	TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20
4	G, H (C, F)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, D: 1 - 147 (UniProt: P51668)

PDB	External Database	Details
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Leu -1	-	expression tag
Glu 0	-	expression tag

B, E: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C, F: 592 - 635 (UniProt: P21580)

PDB	External Database	Details
Gly 587	-	expression tag
Ser 588	-	expression tag
Pro 589	-	expression tag
Glu 590	-	expression tag
Phe 591	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	63109.9
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	63240.7

*Water molecules are not included.