3OJ4
Crystal structure of the A20 ZnF4, ubiquitin and UbcH5A complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 102.646, 102.646, 112.686 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.400 |
R-factor | 0.28381 |
Rwork | 0.280 |
R-free | 0.31882 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1UBQ 1x23 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.013 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 9637 | |
<I/σ(I)> | 16.8 | 3.3 |
Completeness [%] | 98.2 | 99.8 |
Redundancy | 6.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.1 | 292 | 0.1 M HEPES pH 7.1 and 1.85 M Na Malonate pH 7.0, VAPOR DIFFUSION, temperature 292K |