3OJ4
Crystal structure of the A20 ZnF4, ubiquitin and UbcH5A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 102.646, 102.646, 112.686 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.400 |
| R-factor | 0.28381 |
| Rwork | 0.280 |
| R-free | 0.31882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1UBQ 1x23 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.013 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 9637 | |
| <I/σ(I)> | 16.8 | 3.3 |
| Completeness [%] | 98.2 | 99.8 |
| Redundancy | 6.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.1 | 292 | 0.1 M HEPES pH 7.1 and 1.85 M Na Malonate pH 7.0, VAPOR DIFFUSION, temperature 292K |
