3OGO

Structure of the GFP:GFP-nanobody complex at 2.8 A resolution in spacegroup P21212

Replaces:  3MIQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Green fluorescent protein	polymer	249	28087.6	4	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	E, F, G, H	GFP-nanobody	polymer	123	13817.3	4		CAMELUS DROMEDARIUS
3	A, D, F	ISOPROPYL ALCOHOL	non-polymer		60.1	3	Chemie (IPA)
4		water	water		18.0	312	Chemie (HOH)

Sequence modifications

A, B, C, D: -10 - 238 (UniProt: P42212)

PDB	External Database	Details
Ala -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Val 1	-	expression tag
Leu 64	Phe 64	engineered mutation
Cro 65	Ser 65	chromophore
Cro 65	Tyr 66	chromophore
Cro 65	Gly 67	chromophore
Leu 231	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	167619.7
Non-Polymers*	Number of molecules	3
	Total formula weight	180.3
All*	Total formula weight	167800.0

*Water molecules are not included.