3OGO
Structure of the GFP:GFP-nanobody complex at 2.8 A resolution in spacegroup P21212
Replaces: 3MIQExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 140.100, 147.600, 101.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.432 - 2.800 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.25290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.175 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.430 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | |
Number of reflections | 48206 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 91.8 | 73 |
Redundancy | 4.5 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20% PEG 4000, 20% isopropanol, 0.1M trisodium citrate dihydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |