3H7H

Crystal structure of the human transcription elongation factor DSIF, hSpt4/hSpt5 (176-273)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Transcription elongation factor SPT4	polymer	120	13395.4	1	UniProt (P63272) Pfam (PF06093)	Homo sapiens (human)	hSPT4, DRB sensitivity-inducing factor small subunit, DSIF small subunit, DSIF p14
2	B (B)	Transcription elongation factor SPT5	polymer	106	11954.9	1	UniProt (O00267) Pfam (PF03439)	Homo sapiens (human)	hSPT5, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, Tat-cotransactivator 1 protein, Tat-CT1 protein
3	C (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E, F (A, B)	water	water		18.0	196	Chemie (HOH)

Sequence modifications

A: 2 - 117 (UniProt: P63272)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag

B: 176 - 273 (UniProt: O00267)

PDB	External Database	Details
Gly 172	-	expression tag
Ser 173	-	expression tag
His 174	-	expression tag
Met 175	-	expression tag
Gly 274	-	expression tag
Ser 275	-	expression tag
Gly 276	-	expression tag
Cys 277	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25350.3
Non-Polymers*	Number of molecules	2
	Total formula weight	143.5
All*	Total formula weight	25493.8

*Water molecules are not included.