3H7H
Crystal structure of the human transcription elongation factor DSIF, hSpt4/hSpt5 (176-273)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.283 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.096, 52.091, 97.099 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.110 - 1.550 |
Rwork | 0.186 |
R-free | 0.22200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.277 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.550 |
Number of reflections | 28930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | Ammonium sulfate, PEG 3350, Tris/HCl, beta-mercaptoethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |