3EM1

Crystal structure of the mimivirus NDK +Kpn-N62L double mutant complexed with dTDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Nucleoside diphosphate kinase	polymer	146	16627.8	2	UniProt (Q5UQL3) Pfam (PF00334) In PDB	Acanthamoeba polyphaga mimivirus	NDP kinase, NDK
2	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	A, B	THYMIDINE-5'-DIPHOSPHATE	non-polymer		402.2	2	Chemie (TYD)
4		water	water		18.0	245	Chemie (HOH)

Sequence modifications

A, B: 2 - 141 (UniProt: Q5UQL3)

PDB	External Database	Details
Tyr -4	-	expression tag
Lys -3	-	expression tag
Lys -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag
Leu 1	-	expression tag
Leu 62	Asn 62	engineered mutation
Thr 92	-	SEE REMARK 999
Asn 93	-	SEE REMARK 999
Pro 94	-	SEE REMARK 999
Leu 95	-	SEE REMARK 999
Ala 96	Ile 92	SEE REMARK 999
Ser 97	Leu 93	SEE REMARK 999
Ala 98	Thr 94	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33255.7
Non-Polymers*	Number of molecules	4
	Total formula weight	853.0
All*	Total formula weight	34108.7

*Water molecules are not included.