3EM1
Crystal structure of the mimivirus NDK +Kpn-N62L double mutant complexed with dTDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9205 |
Spacegroup name | P 63 |
Unit cell lengths | 70.343, 70.343, 104.137 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.235 - 1.500 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b8p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.432 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.971 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 46603 | |
<I/σ(I)> | 6 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 1M sodium citrate, 0.1M Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |