3AB4

Crystal structure of feedback inhibition resistant mutant of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Aspartokinase	polymer	421	44858.6	8	UniProt (P26512) Pfam (PF00696) Pfam (PF22468) In PDB	Corynebacterium glutamicum (Brevibacterium flavum)	Aspartate kinase
2	B, D, F, H, J...	Aspartokinase	polymer	178	19444.8	8	UniProt (P26512) Pfam (PF22468) In PDB	Corynebacterium glutamicum (Brevibacterium flavum)	Aspartate kinase
3	H, I, J, K, L...	THREONINE	non-polymer		119.1	16	Chemie (THR)
4	G, K, M, O, A...	LYSINE	non-polymer		147.2	7	Chemie (LYS)
5		water	water		18.0	387	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K, M, O: 1 - 421 (UniProt: P26512)

PDB	External Database	Details
Phe 301	Ser 301	SEE REMARK 999

B, D, F, H, J, L, N, P: 2 - 172 (UniProt: P26512)

PDB	External Database	Details
Met 1	-	initiating methionine
Phe 52	Ser 301	SEE REMARK 999
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	514427.8
Non-Polymers*	Number of molecules	23
	Total formula weight	2936.3
All*	Total formula weight	517364.0

*Water molecules are not included.