3AB4
Crystal structure of feedback inhibition resistant mutant of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-03-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 99.033, 112.874, 120.009 |
| Unit cell angles | 76.03, 71.07, 74.50 |
Refinement procedure
| Resolution | 39.740 - 2.470 |
| R-factor | 0.22621 |
| Rwork | 0.223 |
| R-free | 0.28769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aaw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.045 | |
| Number of reflections | 166233 | |
| <I/σ(I)> | 18.5 | 1.6 |
| Completeness [%] | 96.3 | 93.9 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% PEG 4000, 0.05M Sodium citrate, 0.1M Ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
