3AB4
Crystal structure of feedback inhibition resistant mutant of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-03-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 99.033, 112.874, 120.009 |
Unit cell angles | 76.03, 71.07, 74.50 |
Refinement procedure
Resolution | 39.740 - 2.470 |
R-factor | 0.22621 |
Rwork | 0.223 |
R-free | 0.28769 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aaw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.045 | |
Number of reflections | 166233 | |
<I/σ(I)> | 18.5 | 1.6 |
Completeness [%] | 96.3 | 93.9 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% PEG 4000, 0.05M Sodium citrate, 0.1M Ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |