3AB3

Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Guanine nucleotide-binding protein G(k) subunit alpha, Guanine nucleotide-binding protein subunit alpha-13	polymer	362	42363.4	2	UniProt (Q9DC51) UniProt (P27601) Pfam (PF00503)	Mus musculus (mouse)	G-protein subunit alpha-13, G alpha-13
2	B, D (B, D)	Rho guanine nucleotide exchange factor 1	polymer	246	27648.4	2	UniProt (Q92888) Pfam (PF09128)	Homo sapiens (human)	115 kDa guanine nucleotide exchange factor, p115-RhoGEF, p115RhoGEF, Sub1.5
3	E, H (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, I (A, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
5	G, J (A, C)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	2	Chemie (ALF)
6	K, L, M, N (A, B, C, D)	water	water		18.0	87	Chemie (HOH)

Sequence modifications

A, C: 16 - 43 (UniProt: Q9DC51)

PDB	External Database	Details
Arg 44	-	linker
Ser 45	-	linker
Ala 46	-	linker

A, C: 47 - 377 (UniProt: P27601)
B, D: 1 - 233 (UniProt: Q92888)

PDB	External Database	Details
Gly -12	-	expression tag
Ala -11	-	expression tag
Met -10	-	expression tag
Gly -9	-	expression tag
Ile -8	-	expression tag
Gln -7	-	expression tag
Cys -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ile -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	140023.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1141.0
All*	Total formula weight	141164.7

*Water molecules are not included.