3AB3

Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	50.481, 70.640, 88.078
Unit cell angles	77.77, 84.45, 80.11

Refinement procedure

Resolution	48.880 - 2.400
R-factor	0.205
Rwork	0.205
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iap 1zbc
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	37777
<I/σ(I)>	9	2.3
Completeness [%]	81.8	82.1
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% PEG 4000, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K