3AB3

Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001664	molecular_function	G protein-coupled receptor binding
A	0003924	molecular_function	GTPase activity
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007266	biological_process	Rho protein signal transduction
A	0019001	molecular_function	guanyl nucleotide binding
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0005085	molecular_function	guanyl-nucleotide exchange factor activity
B	0005737	cellular_component	cytoplasm
C	0001664	molecular_function	G protein-coupled receptor binding
C	0003924	molecular_function	GTPase activity
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007266	biological_process	Rho protein signal transduction
C	0019001	molecular_function	guanyl nucleotide binding
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
D	0005085	molecular_function	guanyl-nucleotide exchange factor activity
D	0005737	cellular_component	cytoplasm

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 501

Chain	Residue
A	SER62
A	THR203
A	GDP401
A	HOH408
A	HOH409
A	ALF601

---

site_id	AC2
Number of Residues	21
Details	BINDING SITE FOR RESIDUE GDP A 401

Chain	Residue
A	LYS61
A	SER62
A	THR63
A	SER173
A	LEU197
A	LEU198
A	ARG200
A	ASN291
A	LYS292
A	ASP294
A	LEU295
A	THR348
A	ALA349
A	ILE350
A	HOH381
A	HOH408
A	MG501
A	ALF601
A	GLU58
A	SER59
A	GLY60

---

site_id	AC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF A 601

Chain	Residue
A	GLY57
A	GLU58
A	LYS61
A	ARG200
A	PRO202
A	THR203
A	VAL223
A	GLY224
A	GLY225
A	GLN226
A	GDP401
A	HOH408
A	HOH409
A	MG501

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG C 502

Chain	Residue
C	HOH1
C	SER62
C	THR203
C	ASP222
C	HOH388
C	GDP402
C	ALF602

---

site_id	AC5
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDP C 402

Chain	Residue
C	HOH1
C	GLU58
C	SER59
C	GLY60
C	LYS61
C	SER62
C	THR63
C	SER173
C	LEU197
C	LEU198
C	ARG200
C	ASN291
C	LYS292
C	ASP294
C	LEU295
C	THR348
C	ALA349
C	ILE350
C	MG502
C	ALF602

---

site_id	AC6
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ALF C 602

Chain	Residue
C	HOH1
C	GLY57
C	GLU58
C	LYS61
C	ARG200
C	PRO202
C	THR203
C	VAL223
C	GLY224
C	GLY225
C	GLN226
C	HOH388
C	GDP402
C	MG502

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	LIPID: N-myristoyl glycine => ECO:0000250|UniProtKB:P08754

Chain	Residue	Details
A	GLY17
C	GLY17

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	LIPID: S-palmitoyl cysteine => ECO:0000250|UniProtKB:Q14344

Chain	Residue	Details
A	CYS18
C	CYS18

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:16388592, ECO:0000269|PubMed:18940608, ECO:0007744|PDB:1ZCB, ECO:0007744|PDB:3CX6, ECO:0007744|PDB:3CX7

Chain	Residue	Details
A	GLU58
A	LEU197
A	ASN291
A	ALA349
C	GLU58
C	LEU197
C	ASN291
C	ALA349

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:18940608, ECO:0007744|PDB:3CX6

Chain	Residue	Details
A	SER62
A	THR203
C	SER62
C	THR203

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:18940608, ECO:0007744|PDB:3CX7

Chain	Residue	Details
A	SER173
C	SER173

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:Q14344

Chain	Residue	Details
A	THR203
C	THR203

---