3AB3
Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001664 | molecular_function | G protein-coupled receptor binding |
| A | 0003924 | molecular_function | GTPase activity |
| A | 0007165 | biological_process | signal transduction |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007266 | biological_process | Rho protein signal transduction |
| A | 0019001 | molecular_function | guanyl nucleotide binding |
| A | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| B | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| B | 0005737 | cellular_component | cytoplasm |
| C | 0001664 | molecular_function | G protein-coupled receptor binding |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0007165 | biological_process | signal transduction |
| C | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| C | 0007266 | biological_process | Rho protein signal transduction |
| C | 0019001 | molecular_function | guanyl nucleotide binding |
| C | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| D | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| D | 0005737 | cellular_component | cytoplasm |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 501 |
| Chain | Residue |
| A | SER62 |
| A | THR203 |
| A | GDP401 |
| A | HOH408 |
| A | HOH409 |
| A | ALF601 |
| site_id | AC2 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE GDP A 401 |
| Chain | Residue |
| A | LYS61 |
| A | SER62 |
| A | THR63 |
| A | SER173 |
| A | LEU197 |
| A | LEU198 |
| A | ARG200 |
| A | ASN291 |
| A | LYS292 |
| A | ASP294 |
| A | LEU295 |
| A | THR348 |
| A | ALA349 |
| A | ILE350 |
| A | HOH381 |
| A | HOH408 |
| A | MG501 |
| A | ALF601 |
| A | GLU58 |
| A | SER59 |
| A | GLY60 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE ALF A 601 |
| Chain | Residue |
| A | GLY57 |
| A | GLU58 |
| A | LYS61 |
| A | ARG200 |
| A | PRO202 |
| A | THR203 |
| A | VAL223 |
| A | GLY224 |
| A | GLY225 |
| A | GLN226 |
| A | GDP401 |
| A | HOH408 |
| A | HOH409 |
| A | MG501 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG C 502 |
| Chain | Residue |
| C | HOH1 |
| C | SER62 |
| C | THR203 |
| C | ASP222 |
| C | HOH388 |
| C | GDP402 |
| C | ALF602 |
| site_id | AC5 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE GDP C 402 |
| Chain | Residue |
| C | HOH1 |
| C | GLU58 |
| C | SER59 |
| C | GLY60 |
| C | LYS61 |
| C | SER62 |
| C | THR63 |
| C | SER173 |
| C | LEU197 |
| C | LEU198 |
| C | ARG200 |
| C | ASN291 |
| C | LYS292 |
| C | ASP294 |
| C | LEU295 |
| C | THR348 |
| C | ALA349 |
| C | ILE350 |
| C | MG502 |
| C | ALF602 |
| site_id | AC6 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE ALF C 602 |
| Chain | Residue |
| C | HOH1 |
| C | GLY57 |
| C | GLU58 |
| C | LYS61 |
| C | ARG200 |
| C | PRO202 |
| C | THR203 |
| C | VAL223 |
| C | GLY224 |
| C | GLY225 |
| C | GLN226 |
| C | HOH388 |
| C | GDP402 |
| C | MG502 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 26 |
| Details | Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 14 |
| Details | Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 10 |
| Details | Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 22 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"16388592","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18940608","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1ZCB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CX7","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 50 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P08754","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q14344","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
