2ZNV

Crystal structure of human AMSH-LP DUB domain in complex with Lys63-linked ubiquitin dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	AMSH-like protease	polymer	178	19916.2	2	UniProt (Q96FJ0) Pfam (PF01398)	Homo sapiens (human)	AMSH-LP, STAM-binding protein-like 1
2	B, E (B, E)	Ubiquitin	polymer	76	8604.8	2	UniProt (P62991) Pfam (PF00240) UniProt (by SIFTS) (P0CG50)	Mus musculus (mouse)
3	C, F (C, F)	Ubiquitin	polymer	77	8691.9	2	UniProt (P62991) Pfam (PF00240) UniProt (by SIFTS) (P0CG50)	Mus musculus (mouse)
4	G, J (A, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	H, I, K (A, B, D)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
6	L, M, N, O, P... (A, B, C, D, E...)	water	water		18.0	605	Chemie (HOH)

Sequence modifications

A, D: 264 - 436 (UniProt: Q96FJ0)

PDB	External Database	Details
Gly 259	-	expression tag
Pro 260	-	expression tag
Gly 261	-	expression tag
His 262	-	expression tag
Met 263	-	expression tag
Ala 292	Glu 292	engineered mutation

B, E: 1 - 76 (UniProt: P62991)

PDB	External Database	Details
Arg 63	Lys 63	engineered mutation

C, F: 1 - 76 (UniProt: P62991)

PDB	External Database	Details
Asp 77	-	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	74426.0
Non-Polymers*	Number of molecules	5
	Total formula weight	317.0
All*	Total formula weight	74743.0

*Water molecules are not included.