2ZNV
Crystal structure of human AMSH-LP DUB domain in complex with Lys63-linked ubiquitin dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.089, 97.363, 87.894 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 32.470 - 1.600 |
| R-factor | 0.18511 |
| Rwork | 0.183 |
| R-free | 0.21522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2ZNR and 1ubq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.040 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.267 |
| Number of reflections | 83683 | |
| <I/σ(I)> | 16.9 | 3.2 |
| Completeness [%] | 97.3 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 180mM tri-ammonium citrate (pH 7.0), 24% PEG 3350, 3% 1,6-Hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
