Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8T75

Crystal Structure of KRAS4a (GMPPNP) in complex with RAF1 (RBD-CRD)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000165	biological_process	MAPK cascade
A	0001934	biological_process	positive regulation of protein phosphorylation
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005741	cellular_component	mitochondrial outer membrane
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0007165	biological_process	signal transduction
A	0007265	biological_process	Ras protein signal transduction
A	0008283	biological_process	cell population proliferation
A	0008542	biological_process	visual learning
A	0009898	cellular_component	cytoplasmic side of plasma membrane
A	0010467	biological_process	gene expression
A	0010628	biological_process	positive regulation of gene expression
A	0012505	cellular_component	endomembrane system
A	0014009	biological_process	glial cell proliferation
A	0016020	cellular_component	membrane
A	0016601	biological_process	Rac protein signal transduction
A	0016787	molecular_function	hydrolase activity
A	0019003	molecular_function	GDP binding
A	0021897	biological_process	forebrain astrocyte development
A	0030036	biological_process	actin cytoskeleton organization
A	0030857	biological_process	negative regulation of epithelial cell differentiation
A	0032228	biological_process	regulation of synaptic transmission, GABAergic
A	0035022	biological_process	positive regulation of Rac protein signal transduction
A	0035914	biological_process	skeletal muscle cell differentiation
A	0043495	molecular_function	protein-membrane adaptor activity
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0044877	molecular_function	protein-containing complex binding
A	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
A	0048873	biological_process	homeostasis of number of cells within a tissue
A	0051146	biological_process	striated muscle cell differentiation
A	0051402	biological_process	neuron apoptotic process
A	0060252	biological_process	positive regulation of glial cell proliferation
A	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
A	0060509	biological_process	type I pneumocyte differentiation
B	0007165	biological_process	signal transduction
C	0000139	cellular_component	Golgi membrane
C	0000165	biological_process	MAPK cascade
C	0001934	biological_process	positive regulation of protein phosphorylation
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005741	cellular_component	mitochondrial outer membrane
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0007165	biological_process	signal transduction
C	0007265	biological_process	Ras protein signal transduction
C	0008283	biological_process	cell population proliferation
C	0008542	biological_process	visual learning
C	0009898	cellular_component	cytoplasmic side of plasma membrane
C	0010467	biological_process	gene expression
C	0010628	biological_process	positive regulation of gene expression
C	0012505	cellular_component	endomembrane system
C	0014009	biological_process	glial cell proliferation
C	0016020	cellular_component	membrane
C	0016601	biological_process	Rac protein signal transduction
C	0016787	molecular_function	hydrolase activity
C	0019003	molecular_function	GDP binding
C	0021897	biological_process	forebrain astrocyte development
C	0030036	biological_process	actin cytoskeleton organization
C	0030857	biological_process	negative regulation of epithelial cell differentiation
C	0032228	biological_process	regulation of synaptic transmission, GABAergic
C	0035022	biological_process	positive regulation of Rac protein signal transduction
C	0035914	biological_process	skeletal muscle cell differentiation
C	0043495	molecular_function	protein-membrane adaptor activity
C	0043524	biological_process	negative regulation of neuron apoptotic process
C	0044877	molecular_function	protein-containing complex binding
C	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
C	0048873	biological_process	homeostasis of number of cells within a tissue
C	0051146	biological_process	striated muscle cell differentiation
C	0051402	biological_process	neuron apoptotic process
C	0060252	biological_process	positive regulation of glial cell proliferation
C	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
C	0060509	biological_process	type I pneumocyte differentiation
D	0007165	biological_process	signal transduction
E	0000139	cellular_component	Golgi membrane
E	0000165	biological_process	MAPK cascade
E	0001934	biological_process	positive regulation of protein phosphorylation
E	0003924	molecular_function	GTPase activity
E	0003925	molecular_function	G protein activity
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005741	cellular_component	mitochondrial outer membrane
E	0005789	cellular_component	endoplasmic reticulum membrane
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005925	cellular_component	focal adhesion
E	0007165	biological_process	signal transduction
E	0007265	biological_process	Ras protein signal transduction
E	0008283	biological_process	cell population proliferation
E	0008542	biological_process	visual learning
E	0009898	cellular_component	cytoplasmic side of plasma membrane
E	0010467	biological_process	gene expression
E	0010628	biological_process	positive regulation of gene expression
E	0012505	cellular_component	endomembrane system
E	0014009	biological_process	glial cell proliferation
E	0016020	cellular_component	membrane
E	0016601	biological_process	Rac protein signal transduction
E	0016787	molecular_function	hydrolase activity
E	0019003	molecular_function	GDP binding
E	0021897	biological_process	forebrain astrocyte development
E	0030036	biological_process	actin cytoskeleton organization
E	0030857	biological_process	negative regulation of epithelial cell differentiation
E	0032228	biological_process	regulation of synaptic transmission, GABAergic
E	0035022	biological_process	positive regulation of Rac protein signal transduction
E	0035914	biological_process	skeletal muscle cell differentiation
E	0043495	molecular_function	protein-membrane adaptor activity
E	0043524	biological_process	negative regulation of neuron apoptotic process
E	0044877	molecular_function	protein-containing complex binding
E	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
E	0048873	biological_process	homeostasis of number of cells within a tissue
E	0051146	biological_process	striated muscle cell differentiation
E	0051402	biological_process	neuron apoptotic process
E	0060252	biological_process	positive regulation of glial cell proliferation
E	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
E	0060509	biological_process	type I pneumocyte differentiation
F	0007165	biological_process	signal transduction
G	0000139	cellular_component	Golgi membrane
G	0000165	biological_process	MAPK cascade
G	0001934	biological_process	positive regulation of protein phosphorylation
G	0003924	molecular_function	GTPase activity
G	0003925	molecular_function	G protein activity
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0005741	cellular_component	mitochondrial outer membrane
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005925	cellular_component	focal adhesion
G	0007165	biological_process	signal transduction
G	0007265	biological_process	Ras protein signal transduction
G	0008283	biological_process	cell population proliferation
G	0008542	biological_process	visual learning
G	0009898	cellular_component	cytoplasmic side of plasma membrane
G	0010467	biological_process	gene expression
G	0010628	biological_process	positive regulation of gene expression
G	0012505	cellular_component	endomembrane system
G	0014009	biological_process	glial cell proliferation
G	0016020	cellular_component	membrane
G	0016601	biological_process	Rac protein signal transduction
G	0016787	molecular_function	hydrolase activity
G	0019003	molecular_function	GDP binding
G	0021897	biological_process	forebrain astrocyte development
G	0030036	biological_process	actin cytoskeleton organization
G	0030857	biological_process	negative regulation of epithelial cell differentiation
G	0032228	biological_process	regulation of synaptic transmission, GABAergic
G	0035022	biological_process	positive regulation of Rac protein signal transduction
G	0035914	biological_process	skeletal muscle cell differentiation
G	0043495	molecular_function	protein-membrane adaptor activity
G	0043524	biological_process	negative regulation of neuron apoptotic process
G	0044877	molecular_function	protein-containing complex binding
G	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
G	0048873	biological_process	homeostasis of number of cells within a tissue
G	0051146	biological_process	striated muscle cell differentiation
G	0051402	biological_process	neuron apoptotic process
G	0060252	biological_process	positive regulation of glial cell proliferation
G	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
G	0060509	biological_process	type I pneumocyte differentiation
H	0007165	biological_process	signal transduction

Functional Information from PROSITE/UniProt

site_id	PS00479
Number of Residues	46
Details	ZF_DAG_PE_1 Zinc finger phorbol-ester/DAG-type signature. HnFarktflklaf.CdiCqkfLlngfr.....CqtCgykfHehCstkvptm..C

Chain	Residue	Details
B	HIS139-CYS184

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	184
Details	ZN_FING: Phorbol-ester/DAG-type => ECO:0000255\|PROSITE-ProRule:PRU00226

Chain	Residue	Details
B	THR138-CYS184
E	VAL29
E	ALA59
E	ASN116
G	GLY10
G	VAL29
G	ALA59
G	ASN116
D	THR138-CYS184
F	THR138-CYS184
H	THR138-CYS184
C	GLY10
C	VAL29
C	ALA59
C	ASN116
E	GLY10

site_id	SWS_FT_FI2
Number of Residues	32
Details	BINDING:

Chain	Residue	Details
B	HIS139
D	CYS152
D	CYS155
D	CYS165
D	CYS168
D	HIS173
D	CYS176
D	CYS184
F	HIS139
F	CYS152
F	CYS155
B	CYS152
F	CYS165
F	CYS168
F	HIS173
F	CYS176
F	CYS184
H	HIS139
H	CYS152
H	CYS155
H	CYS165
H	CYS168
B	CYS155
H	HIS173
H	CYS176
H	CYS184
B	CYS165
B	CYS168
B	HIS173
B	CYS176
B	CYS184
D	HIS139

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269\|Ref.17

Chain	Residue	Details
A	THR2
C	THR2
E	THR2
G	THR2

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:22711838

Chain	Residue	Details
A	LYS104
C	LYS104
E	LYS104
G	LYS104

site_id	SWS_FT_FI5
Number of Residues	4
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269\|PubMed:19744486

Chain	Residue	Details
A	THR35
C	THR35
E	THR35
G	THR35

site_id	SWS_FT_FI6
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:30442762

Chain	Residue	Details
A	LYS170
C	LYS170
E	LYS170
G	LYS170

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)