8T75

Crystal Structure of KRAS4a (GMPPNP) in complex with RAF1 (RBD-CRD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	104.409, 199.509, 113.428
Unit cell angles	90.00, 112.28, 90.00

Refinement procedure

Resolution	46.450 - 2.650
R-factor	0.179
Rwork	0.178
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.378
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.450	46.450	2.810
High resolution limit [Å]	2.650	7.860	2.650
Rmerge	0.082	0.029	0.833
Rmeas	0.098	0.034	0.988
Number of reflections	61031	2392	9814
<I/σ(I)>	10.73
Completeness [%]	98.3
Redundancy	3.3
CC(1/2)	0.997	0.999	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate (pH 4.6), 2 M sodium formate