8C06

Structure of Dimeric HECT E3 Ubiquitin Ligase UBR5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000209	biological_process	protein polyubiquitination
A	0000785	cellular_component	chromatin
A	0003723	molecular_function	RNA binding
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006974	biological_process	DNA damage response
A	0006979	biological_process	response to oxidative stress
A	0008270	molecular_function	zinc ion binding
A	0010498	biological_process	proteasomal protein catabolic process
A	0010628	biological_process	positive regulation of gene expression
A	0016020	cellular_component	membrane
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0030520	biological_process	estrogen receptor signaling pathway
A	0032991	cellular_component	protein-containing complex
A	0033696	biological_process	heterochromatin boundary formation
A	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
A	0035519	biological_process	protein K29-linked ubiquitination
A	0042307	biological_process	positive regulation of protein import into nucleus
A	0043130	molecular_function	ubiquitin binding
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045879	biological_process	negative regulation of smoothened signaling pathway
A	0046872	molecular_function	metal ion binding
A	0048384	biological_process	retinoic acid receptor signaling pathway
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050847	biological_process	progesterone receptor signaling pathway
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0070561	biological_process	vitamin D receptor signaling pathway
A	0070936	biological_process	protein K48-linked ubiquitination
A	0070979	biological_process	protein K11-linked ubiquitination
A	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
A	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
A	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
A	0140455	biological_process	cytoplasm protein quality control
A	0140861	biological_process	DNA repair-dependent chromatin remodeling
A	0141198	biological_process	protein branched polyubiquitination
B	0000209	biological_process	protein polyubiquitination
B	0000785	cellular_component	chromatin
B	0003723	molecular_function	RNA binding
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006281	biological_process	DNA repair
B	0006974	biological_process	DNA damage response
B	0006979	biological_process	response to oxidative stress
B	0008270	molecular_function	zinc ion binding
B	0010498	biological_process	proteasomal protein catabolic process
B	0010628	biological_process	positive regulation of gene expression
B	0016020	cellular_component	membrane
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0030520	biological_process	estrogen receptor signaling pathway
B	0032991	cellular_component	protein-containing complex
B	0033696	biological_process	heterochromatin boundary formation
B	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
B	0035519	biological_process	protein K29-linked ubiquitination
B	0042307	biological_process	positive regulation of protein import into nucleus
B	0043130	molecular_function	ubiquitin binding
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0045879	biological_process	negative regulation of smoothened signaling pathway
B	0046872	molecular_function	metal ion binding
B	0048384	biological_process	retinoic acid receptor signaling pathway
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050847	biological_process	progesterone receptor signaling pathway
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0070561	biological_process	vitamin D receptor signaling pathway
B	0070936	biological_process	protein K48-linked ubiquitination
B	0070979	biological_process	protein K11-linked ubiquitination
B	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
B	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
B	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
B	0140455	biological_process	cytoplasm protein quality control
B	0140861	biological_process	DNA repair-dependent chromatin remodeling
B	0141198	biological_process	protein branched polyubiquitination
D	0000209	biological_process	protein polyubiquitination
D	0000785	cellular_component	chromatin
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006974	biological_process	DNA damage response
D	0006979	biological_process	response to oxidative stress
D	0008270	molecular_function	zinc ion binding
D	0010498	biological_process	proteasomal protein catabolic process
D	0010628	biological_process	positive regulation of gene expression
D	0016020	cellular_component	membrane
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0030520	biological_process	estrogen receptor signaling pathway
D	0032991	cellular_component	protein-containing complex
D	0033696	biological_process	heterochromatin boundary formation
D	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
D	0035519	biological_process	protein K29-linked ubiquitination
D	0042307	biological_process	positive regulation of protein import into nucleus
D	0043130	molecular_function	ubiquitin binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0045879	biological_process	negative regulation of smoothened signaling pathway
D	0046872	molecular_function	metal ion binding
D	0048384	biological_process	retinoic acid receptor signaling pathway
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050847	biological_process	progesterone receptor signaling pathway
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0070561	biological_process	vitamin D receptor signaling pathway
D	0070936	biological_process	protein K48-linked ubiquitination
D	0070979	biological_process	protein K11-linked ubiquitination
D	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
D	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
D	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
D	0140455	biological_process	cytoplasm protein quality control
D	0140861	biological_process	DNA repair-dependent chromatin remodeling
D	0141198	biological_process	protein branched polyubiquitination
E	0000209	biological_process	protein polyubiquitination
E	0000785	cellular_component	chromatin
E	0003723	molecular_function	RNA binding
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006974	biological_process	DNA damage response
E	0006979	biological_process	response to oxidative stress
E	0008270	molecular_function	zinc ion binding
E	0010498	biological_process	proteasomal protein catabolic process
E	0010628	biological_process	positive regulation of gene expression
E	0016020	cellular_component	membrane
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0030520	biological_process	estrogen receptor signaling pathway
E	0032991	cellular_component	protein-containing complex
E	0033696	biological_process	heterochromatin boundary formation
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0035519	biological_process	protein K29-linked ubiquitination
E	0042307	biological_process	positive regulation of protein import into nucleus
E	0043130	molecular_function	ubiquitin binding
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0045879	biological_process	negative regulation of smoothened signaling pathway
E	0046872	molecular_function	metal ion binding
E	0048384	biological_process	retinoic acid receptor signaling pathway
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050847	biological_process	progesterone receptor signaling pathway
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0070561	biological_process	vitamin D receptor signaling pathway
E	0070936	biological_process	protein K48-linked ubiquitination
E	0070979	biological_process	protein K11-linked ubiquitination
E	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
E	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
E	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
E	0140455	biological_process	cytoplasm protein quality control
E	0140861	biological_process	DNA repair-dependent chromatin remodeling
E	0141198	biological_process	protein branched polyubiquitination
F	0000209	biological_process	protein polyubiquitination
F	0000785	cellular_component	chromatin
F	0003723	molecular_function	RNA binding
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006281	biological_process	DNA repair
F	0006974	biological_process	DNA damage response
F	0006979	biological_process	response to oxidative stress
F	0008270	molecular_function	zinc ion binding
F	0010498	biological_process	proteasomal protein catabolic process
F	0010628	biological_process	positive regulation of gene expression
F	0016020	cellular_component	membrane
F	0016567	biological_process	protein ubiquitination
F	0016740	molecular_function	transferase activity
F	0030520	biological_process	estrogen receptor signaling pathway
F	0032991	cellular_component	protein-containing complex
F	0033696	biological_process	heterochromatin boundary formation
F	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
F	0035519	biological_process	protein K29-linked ubiquitination
F	0042307	biological_process	positive regulation of protein import into nucleus
F	0043130	molecular_function	ubiquitin binding
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045879	biological_process	negative regulation of smoothened signaling pathway
F	0046872	molecular_function	metal ion binding
F	0048384	biological_process	retinoic acid receptor signaling pathway
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050847	biological_process	progesterone receptor signaling pathway
F	0061630	molecular_function	ubiquitin protein ligase activity
F	0070561	biological_process	vitamin D receptor signaling pathway
F	0070936	biological_process	protein K48-linked ubiquitination
F	0070979	biological_process	protein K11-linked ubiquitination
F	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
F	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
F	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
F	0140455	biological_process	cytoplasm protein quality control
F	0140861	biological_process	DNA repair-dependent chromatin remodeling
F	0141198	biological_process	protein branched polyubiquitination
G	0000209	biological_process	protein polyubiquitination
G	0000785	cellular_component	chromatin
G	0003723	molecular_function	RNA binding
G	0004842	molecular_function	ubiquitin-protein transferase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006281	biological_process	DNA repair
G	0006974	biological_process	DNA damage response
G	0006979	biological_process	response to oxidative stress
G	0008270	molecular_function	zinc ion binding
G	0010498	biological_process	proteasomal protein catabolic process
G	0010628	biological_process	positive regulation of gene expression
G	0016020	cellular_component	membrane
G	0016567	biological_process	protein ubiquitination
G	0016740	molecular_function	transferase activity
G	0030520	biological_process	estrogen receptor signaling pathway
G	0032991	cellular_component	protein-containing complex
G	0033696	biological_process	heterochromatin boundary formation
G	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
G	0035519	biological_process	protein K29-linked ubiquitination
G	0042307	biological_process	positive regulation of protein import into nucleus
G	0043130	molecular_function	ubiquitin binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045879	biological_process	negative regulation of smoothened signaling pathway
G	0046872	molecular_function	metal ion binding
G	0048384	biological_process	retinoic acid receptor signaling pathway
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0050847	biological_process	progesterone receptor signaling pathway
G	0061630	molecular_function	ubiquitin protein ligase activity
G	0070561	biological_process	vitamin D receptor signaling pathway
G	0070936	biological_process	protein K48-linked ubiquitination
G	0070979	biological_process	protein K11-linked ubiquitination
G	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
G	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
G	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
G	0140455	biological_process	cytoplasm protein quality control
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	0141198	biological_process	protein branched polyubiquitination

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	408
Details	ZN_FING: UBR-type => ECO:0000255|PROSITE-ProRule:PRU00508

Chain	Residue	Details
A	ASP1177-ALA1245
B	ASP1177-ALA1245
D	ASP1177-ALA1245
E	ASP1177-ALA1245
F	ASP1177-ALA1245
G	ASP1177-ALA1245

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00104, ECO:0000305|PubMed:11287654, ECO:0000305|PubMed:23027739, ECO:0000305|PubMed:28689657, ECO:0000305|PubMed:37040767, ECO:0000305|PubMed:37478862, ECO:0000305|PubMed:37620400

Chain	Residue	Details
A	CYS2768
B	CYS2768
D	CYS2768
E	CYS2768
F	CYS2768
G	CYS2768

---

site_id	SWS_FT_FI3
Number of Residues	66
Details	BINDING: BINDING => ECO:0000269|PubMed:37040767, ECO:0000269|PubMed:37409633, ECO:0000269|PubMed:37478846, ECO:0000269|PubMed:37620400, ECO:0007744|PDB:8BJA, ECO:0007744|PDB:8C06, ECO:0007744|PDB:8D4X, ECO:0007744|PDB:8E0Q, ECO:0007744|PDB:8EWI, ECO:0007744|PDB:8P82

Chain	Residue	Details
A	CYS1179
A	CYS1234
A	CYS1240
B	CYS1179
B	CYS1196
B	CYS1199
B	CYS1208
B	CYS1211
B	CYS1215
B	HIS1216
B	HIS1219
A	CYS1196
B	CYS1232
B	CYS1234
B	CYS1240
D	CYS1179
D	CYS1196
D	CYS1199
D	CYS1208
D	CYS1211
D	CYS1215
D	HIS1216
A	CYS1199
D	HIS1219
D	CYS1232
D	CYS1234
D	CYS1240
E	CYS1179
E	CYS1196
E	CYS1199
E	CYS1208
E	CYS1211
E	CYS1215
A	CYS1208
E	HIS1216
E	HIS1219
E	CYS1232
E	CYS1234
E	CYS1240
F	CYS1179
F	CYS1196
F	CYS1199
F	CYS1208
F	CYS1211
A	CYS1211
F	CYS1215
F	HIS1216
F	HIS1219
F	CYS1232
F	CYS1234
F	CYS1240
G	CYS1179
G	CYS1196
G	CYS1199
G	CYS1208
A	CYS1215
G	CYS1211
G	CYS1215
G	HIS1216
G	HIS1219
G	CYS1232
G	CYS1234
G	CYS1240
A	HIS1216
A	HIS1219
A	CYS1232

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: N-acetylthreonine => ECO:0007744|PubMed:19413330

Chain	Residue	Details
A	THR2
B	THR2
D	THR2
E	THR2
F	THR2
G	THR2

---

site_id	SWS_FT_FI5
Number of Residues	24
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:21924388, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER110
D	SER578
D	SER612
D	SER808
E	SER110
E	SER578
E	SER612
E	SER808
F	SER110
F	SER578
F	SER612
A	SER578
F	SER808
G	SER110
G	SER578
G	SER612
G	SER808
A	SER612
A	SER808
B	SER110
B	SER578
B	SER612
B	SER808
D	SER110

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:21924388, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER327
B	SER327
D	SER327
E	SER327
F	SER327
G	SER327

---

site_id	SWS_FT_FI7
Number of Residues	84
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:21924388

Chain	Residue	Details
A	SER352
A	SER2026
A	SER2028
A	SER2076
A	SER2289
A	SER2484
B	SER352
B	SER928
B	SER1018
B	SER1227
B	SER1355
A	SER928
B	SER1375
B	SER1481
B	SER1741
B	SER1780
B	SER2026
B	SER2028
B	SER2076
B	SER2289
B	SER2484
D	SER352
A	SER1018
D	SER928
D	SER1018
D	SER1227
D	SER1355
D	SER1375
D	SER1481
D	SER1741
D	SER1780
D	SER2026
D	SER2028
A	SER1227
D	SER2076
D	SER2289
D	SER2484
E	SER352
E	SER928
E	SER1018
E	SER1227
E	SER1355
E	SER1375
E	SER1481
A	SER1355
E	SER1741
E	SER1780
E	SER2026
E	SER2028
E	SER2076
E	SER2289
E	SER2484
F	SER352
F	SER928
F	SER1018
A	SER1375
F	SER1227
F	SER1355
F	SER1375
F	SER1481
F	SER1741
F	SER1780
F	SER2026
F	SER2028
F	SER2076
F	SER2289
A	SER1481
F	SER2484
G	SER352
G	SER928
G	SER1018
G	SER1227
G	SER1355
G	SER1375
G	SER1481
G	SER1741
G	SER1780
A	SER1741
G	SER2026
G	SER2028
G	SER2076
G	SER2289
G	SER2484
A	SER1780

---

site_id	SWS_FT_FI8
Number of Residues	36
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:21924388

Chain	Residue	Details
A	THR637
B	THR1736
B	THR2030
B	THR2213
D	THR637
D	THR1115
D	THR1135
D	THR1736
D	THR2030
D	THR2213
E	THR637
A	THR1115
E	THR1115
E	THR1135
E	THR1736
E	THR2030
E	THR2213
F	THR637
F	THR1115
F	THR1135
F	THR1736
F	THR2030
A	THR1135
F	THR2213
G	THR637
G	THR1115
G	THR1135
G	THR1736
G	THR2030
G	THR2213
A	THR1736
A	THR2030
A	THR2213
B	THR637
B	THR1115
B	THR1135

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:21924388, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER1308
B	SER1308
D	SER1308
E	SER1308
F	SER1308
G	SER1308

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER1549
B	SER1549
D	SER1549
E	SER1549
F	SER1549
G	SER1549

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
A	TYR1746
B	TYR1746
D	TYR1746
E	TYR1746
F	TYR1746
G	TYR1746

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:21924388, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	THR1969
B	THR1969
D	THR1969
E	THR1969
F	THR1969
G	THR1969

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER1990
B	SER1990
D	SER1990
E	SER1990
F	SER1990
G	SER1990

---

site_id	SWS_FT_FI14
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER2241
F	SER2469
G	SER2241
G	SER2469
A	SER2469
B	SER2241
B	SER2469
D	SER2241
D	SER2469
E	SER2241
E	SER2469
F	SER2241

---

site_id	SWS_FT_FI15
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER2486
B	SER2486
D	SER2486
E	SER2486
F	SER2486
G	SER2486

---