8C06

Structure of Dimeric HECT E3 Ubiquitin Ligase UBR5

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000209	biological_process	protein polyubiquitination
A	0000785	cellular_component	chromatin
A	0003723	molecular_function	RNA binding
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006281	biological_process	DNA repair
A	0006974	biological_process	DNA damage response
A	0008270	molecular_function	zinc ion binding
A	0010498	biological_process	proteasomal protein catabolic process
A	0010628	biological_process	positive regulation of gene expression
A	0016020	cellular_component	membrane
A	0016567	biological_process	protein ubiquitination
A	0016740	molecular_function	transferase activity
A	0032991	cellular_component	protein-containing complex
A	0033696	biological_process	heterochromatin boundary formation
A	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
A	0035519	biological_process	protein K29-linked ubiquitination
A	0042307	biological_process	positive regulation of protein import into nucleus
A	0043130	molecular_function	ubiquitin binding
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0045879	biological_process	negative regulation of smoothened signaling pathway
A	0046872	molecular_function	metal ion binding
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050847	biological_process	progesterone receptor signaling pathway
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0070936	biological_process	protein K48-linked ubiquitination
A	0070979	biological_process	protein K11-linked ubiquitination
A	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
A	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
A	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
A	0140455	biological_process	cytoplasm protein quality control
A	0140861	biological_process	DNA repair-dependent chromatin remodeling
A	0141198	biological_process	protein branched polyubiquitination
B	0000209	biological_process	protein polyubiquitination
B	0000785	cellular_component	chromatin
B	0003723	molecular_function	RNA binding
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006281	biological_process	DNA repair
B	0006974	biological_process	DNA damage response
B	0008270	molecular_function	zinc ion binding
B	0010498	biological_process	proteasomal protein catabolic process
B	0010628	biological_process	positive regulation of gene expression
B	0016020	cellular_component	membrane
B	0016567	biological_process	protein ubiquitination
B	0016740	molecular_function	transferase activity
B	0032991	cellular_component	protein-containing complex
B	0033696	biological_process	heterochromatin boundary formation
B	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
B	0035519	biological_process	protein K29-linked ubiquitination
B	0042307	biological_process	positive regulation of protein import into nucleus
B	0043130	molecular_function	ubiquitin binding
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0045879	biological_process	negative regulation of smoothened signaling pathway
B	0046872	molecular_function	metal ion binding
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0050847	biological_process	progesterone receptor signaling pathway
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0070936	biological_process	protein K48-linked ubiquitination
B	0070979	biological_process	protein K11-linked ubiquitination
B	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
B	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
B	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
B	0140455	biological_process	cytoplasm protein quality control
B	0140861	biological_process	DNA repair-dependent chromatin remodeling
B	0141198	biological_process	protein branched polyubiquitination
D	0000209	biological_process	protein polyubiquitination
D	0000785	cellular_component	chromatin
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006281	biological_process	DNA repair
D	0006974	biological_process	DNA damage response
D	0008270	molecular_function	zinc ion binding
D	0010498	biological_process	proteasomal protein catabolic process
D	0010628	biological_process	positive regulation of gene expression
D	0016020	cellular_component	membrane
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0032991	cellular_component	protein-containing complex
D	0033696	biological_process	heterochromatin boundary formation
D	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
D	0035519	biological_process	protein K29-linked ubiquitination
D	0042307	biological_process	positive regulation of protein import into nucleus
D	0043130	molecular_function	ubiquitin binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0045879	biological_process	negative regulation of smoothened signaling pathway
D	0046872	molecular_function	metal ion binding
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0050847	biological_process	progesterone receptor signaling pathway
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0070936	biological_process	protein K48-linked ubiquitination
D	0070979	biological_process	protein K11-linked ubiquitination
D	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
D	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
D	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
D	0140455	biological_process	cytoplasm protein quality control
D	0140861	biological_process	DNA repair-dependent chromatin remodeling
D	0141198	biological_process	protein branched polyubiquitination
E	0000209	biological_process	protein polyubiquitination
E	0000785	cellular_component	chromatin
E	0003723	molecular_function	RNA binding
E	0004842	molecular_function	ubiquitin-protein transferase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006281	biological_process	DNA repair
E	0006974	biological_process	DNA damage response
E	0008270	molecular_function	zinc ion binding
E	0010498	biological_process	proteasomal protein catabolic process
E	0010628	biological_process	positive regulation of gene expression
E	0016020	cellular_component	membrane
E	0016567	biological_process	protein ubiquitination
E	0016740	molecular_function	transferase activity
E	0032991	cellular_component	protein-containing complex
E	0033696	biological_process	heterochromatin boundary formation
E	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
E	0035519	biological_process	protein K29-linked ubiquitination
E	0042307	biological_process	positive regulation of protein import into nucleus
E	0043130	molecular_function	ubiquitin binding
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0045879	biological_process	negative regulation of smoothened signaling pathway
E	0046872	molecular_function	metal ion binding
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0050847	biological_process	progesterone receptor signaling pathway
E	0061630	molecular_function	ubiquitin protein ligase activity
E	0070936	biological_process	protein K48-linked ubiquitination
E	0070979	biological_process	protein K11-linked ubiquitination
E	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
E	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
E	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
E	0140455	biological_process	cytoplasm protein quality control
E	0140861	biological_process	DNA repair-dependent chromatin remodeling
E	0141198	biological_process	protein branched polyubiquitination
F	0000209	biological_process	protein polyubiquitination
F	0000785	cellular_component	chromatin
F	0003723	molecular_function	RNA binding
F	0004842	molecular_function	ubiquitin-protein transferase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006281	biological_process	DNA repair
F	0006974	biological_process	DNA damage response
F	0008270	molecular_function	zinc ion binding
F	0010498	biological_process	proteasomal protein catabolic process
F	0010628	biological_process	positive regulation of gene expression
F	0016020	cellular_component	membrane
F	0016567	biological_process	protein ubiquitination
F	0016740	molecular_function	transferase activity
F	0032991	cellular_component	protein-containing complex
F	0033696	biological_process	heterochromatin boundary formation
F	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
F	0035519	biological_process	protein K29-linked ubiquitination
F	0042307	biological_process	positive regulation of protein import into nucleus
F	0043130	molecular_function	ubiquitin binding
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045879	biological_process	negative regulation of smoothened signaling pathway
F	0046872	molecular_function	metal ion binding
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050847	biological_process	progesterone receptor signaling pathway
F	0061630	molecular_function	ubiquitin protein ligase activity
F	0070936	biological_process	protein K48-linked ubiquitination
F	0070979	biological_process	protein K11-linked ubiquitination
F	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
F	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
F	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
F	0140455	biological_process	cytoplasm protein quality control
F	0140861	biological_process	DNA repair-dependent chromatin remodeling
F	0141198	biological_process	protein branched polyubiquitination
G	0000209	biological_process	protein polyubiquitination
G	0000785	cellular_component	chromatin
G	0003723	molecular_function	RNA binding
G	0004842	molecular_function	ubiquitin-protein transferase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006281	biological_process	DNA repair
G	0006974	biological_process	DNA damage response
G	0008270	molecular_function	zinc ion binding
G	0010498	biological_process	proteasomal protein catabolic process
G	0010628	biological_process	positive regulation of gene expression
G	0016020	cellular_component	membrane
G	0016567	biological_process	protein ubiquitination
G	0016740	molecular_function	transferase activity
G	0032991	cellular_component	protein-containing complex
G	0033696	biological_process	heterochromatin boundary formation
G	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
G	0035519	biological_process	protein K29-linked ubiquitination
G	0042307	biological_process	positive regulation of protein import into nucleus
G	0043130	molecular_function	ubiquitin binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045879	biological_process	negative regulation of smoothened signaling pathway
G	0046872	molecular_function	metal ion binding
G	0048471	cellular_component	perinuclear region of cytoplasm
G	0050847	biological_process	progesterone receptor signaling pathway
G	0061630	molecular_function	ubiquitin protein ligase activity
G	0070936	biological_process	protein K48-linked ubiquitination
G	0070979	biological_process	protein K11-linked ubiquitination
G	0071629	biological_process	cytoplasm protein quality control by the ubiquitin-proteasome system
G	0071630	biological_process	nuclear protein quality control by the ubiquitin-proteasome system
G	0090263	biological_process	positive regulation of canonical Wnt signaling pathway
G	0140455	biological_process	cytoplasm protein quality control
G	0140861	biological_process	DNA repair-dependent chromatin remodeling
G	0141198	biological_process	protein branched polyubiquitination

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	136
Details	Zinc finger: {"description":"UBR-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00508","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Binding site: {"evidences":[{"source":"PubMed","id":"37040767","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37409633","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37478846","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37620400","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8BJA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8C06","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8D4X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8E0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8EWI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8P82","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N-acetylthreonine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	22
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"21924388","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"21924388","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"21924388","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00104","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"11287654","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"23027739","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28689657","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"37040767","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"37478862","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"37620400","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---