7YS6

Cryo-EM structure of the Serotonin 6 (5-HT6) receptor-DNGs-scFv16 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004930	molecular_function	G protein-coupled receptor activity
A	0004993	molecular_function	G protein-coupled serotonin receptor activity
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0016020	cellular_component	membrane
B	0000166	molecular_function	nucleotide binding
B	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
B	0003091	biological_process	renal water homeostasis
B	0003924	molecular_function	GTPase activity
B	0003925	molecular_function	G protein activity
B	0005515	molecular_function	protein binding
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005834	cellular_component	heterotrimeric G-protein complex
B	0005886	cellular_component	plasma membrane
B	0007165	biological_process	signal transduction
B	0007186	biological_process	G protein-coupled receptor signaling pathway
B	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
B	0007190	biological_process	activation of adenylate cyclase activity
B	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
B	0007192	biological_process	adenylate cyclase-activating serotonin receptor signaling pathway
B	0007608	biological_process	sensory perception of smell
B	0010854	molecular_function	adenylate cyclase regulator activity
B	0010856	molecular_function	adenylate cyclase activator activity
B	0014819	biological_process	regulation of skeletal muscle contraction
B	0016020	cellular_component	membrane
B	0016787	molecular_function	hydrolase activity
B	0019001	molecular_function	guanyl nucleotide binding
B	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0031748	molecular_function	D1 dopamine receptor binding
B	0032588	cellular_component	trans-Golgi network membrane
B	0046872	molecular_function	metal ion binding
B	0046907	biological_process	intracellular transport
B	0048589	biological_process	developmental growth
B	0050796	biological_process	regulation of insulin secretion
B	0050890	biological_process	cognition
B	0060348	biological_process	bone development
B	0060789	biological_process	hair follicle placode formation
B	0070062	cellular_component	extracellular exosome
B	0070527	biological_process	platelet aggregation
B	0071377	biological_process	cellular response to glucagon stimulus
B	0071380	biological_process	cellular response to prostaglandin E stimulus
B	0071468	biological_process	cellular response to acidic pH
B	0071870	biological_process	cellular response to catecholamine stimulus
B	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway
B	0097700	biological_process	vascular endothelial cell response to laminar fluid shear stress
B	0120162	biological_process	positive regulation of cold-induced thermogenesis
C	0001750	cellular_component	photoreceptor outer segment
C	0003924	molecular_function	GTPase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
C	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
C	0007265	biological_process	Ras protein signal transduction
C	0008283	biological_process	cell population proliferation
C	0016020	cellular_component	membrane
C	0030159	molecular_function	signaling receptor complex adaptor activity
C	0044877	molecular_function	protein-containing complex binding
C	0045202	cellular_component	synapse
C	0050909	biological_process	sensory perception of taste
C	0051020	molecular_function	GTPase binding
C	0060041	biological_process	retina development in camera-type eye
C	0070062	cellular_component	extracellular exosome
C	0071380	biological_process	cellular response to prostaglandin E stimulus
C	0071870	biological_process	cellular response to catecholamine stimulus
C	0097381	cellular_component	photoreceptor disc membrane
C	1903561	cellular_component	extracellular vesicle
D	0005515	molecular_function	protein binding
D	0005834	cellular_component	heterotrimeric G-protein complex
D	0005886	cellular_component	plasma membrane
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
D	0016020	cellular_component	membrane
D	0031681	molecular_function	G-protein beta-subunit binding
D	0045202	cellular_component	synapse
D	0048144	biological_process	fibroblast proliferation
D	0070062	cellular_component	extracellular exosome
D	0071380	biological_process	cellular response to prostaglandin E stimulus
D	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00237
Number of Residues	17
Details	G_PROTEIN_RECEP_F1_1 G-protein coupled receptors family 1 signature. ASIlNLCLISLDRYLlI

Chain	Residue	Details
A	ALA112-ILE128

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
C	LEU87-SER101
C	ILE174-ILE188
C	LEU302-ALA316

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	9
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	29
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	19
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	26
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	23
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"36989299","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"37327704","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7YS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JLZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"35714614","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7XTB","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	48
Details	Repeat: {"description":"WD 1","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	45
Details	Repeat: {"description":"WD 2","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	40
Details	Repeat: {"description":"WD 3","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	41
Details	Repeat: {"description":"WD 4","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	43
Details	Repeat: {"description":"WD 5","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	41
Details	Repeat: {"description":"WD 6","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	30
Details	Repeat: {"description":"WD 7","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	Modified residue: {"description":"Phosphohistidine","evidences":[{"source":"UniProtKB","id":"P62871","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	13
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	9
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	7
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	5
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	17
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P04896","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)"}

Chain

Residue

Details

---