7SZ7
Cryo-EM structure of the extracellular module of the full-length EGFR bound to TGF-alpha. "tips-juxtaposed" conformation
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004713 | molecular_function | protein tyrosine kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007169 | biological_process | cell surface receptor protein tyrosine kinase signaling pathway |
| A | 0016020 | cellular_component | membrane |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004713 | molecular_function | protein tyrosine kinase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007169 | biological_process | cell surface receptor protein tyrosine kinase signaling pathway |
| B | 0016020 | cellular_component | membrane |
Functional Information from PROSITE/UniProt
| site_id | PS00022 |
| Number of Residues | 12 |
| Details | EGF_1 EGF-like domain signature 1. CvChsGyvGArC |
| Chain | Residue | Details |
| C | CYS32-CYS43 |
| site_id | PS00107 |
| Number of Residues | 28 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGSGAFGTVYkGlwipegekvkip......VAIK |
| Chain | Residue | Details |
| A | LEU694-LYS721 |
| site_id | PS00109 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_TYR Tyrosine protein kinases specific active-site signature. LVHrDLAARNVLV |
| Chain | Residue | Details |
| A | LEU809-VAL821 |
| site_id | PS01186 |
| Number of Residues | 12 |
| Details | EGF_2 EGF-like domain signature 2. CvChsGYvgar....C |
| Chain | Residue | Details |
| C | CYS32-CYS43 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1240 |
| Details | TOPO_DOM: Extracellular => ECO:0000255 |
| Chain | Residue | Details |
| A | LEU1-SER621 | |
| B | LEU1-SER621 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 44 |
| Details | TRANSMEM: Helical => ECO:0000255 |
| Chain | Residue | Details |
| A | ILE622-MET644 | |
| B | ILE622-MET644 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1082 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000255 |
| Chain | Residue | Details |
| A | ARG645-ALA1186 | |
| B | ARG645-ALA1186 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10028 |
| Chain | Residue | Details |
| A | ASP813 | |
| B | ASP813 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:19563760, ECO:0007744|PDB:2GS7, ECO:0007744|PDB:3GT8, ECO:0007744|PDB:4ZSE, ECO:0007744|PDB:5CNN, ECO:0007744|PDB:5CNO, ECO:0007744|PDB:5D41 |
| Chain | Residue | Details |
| A | LEU694 | |
| B | LEU694 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17349580, ECO:0000269|PubMed:19563760, ECO:0007744|PDB:2EB3, ECO:0007744|PDB:2GS7, ECO:0007744|PDB:2ITN, ECO:0007744|PDB:3GT8, ECO:0007744|PDB:3VJN, ECO:0007744|PDB:3VJO, ECO:0007744|PDB:4RIW, ECO:0007744|PDB:4RIY, ECO:0007744|PDB:4ZSE, ECO:0007744|PDB:5CNN, ECO:0007744|PDB:5CNO, ECO:0007744|PDB:5D41 |
| Chain | Residue | Details |
| A | LYS721 | |
| B | LYS721 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17349580, ECO:0000269|PubMed:19563760, ECO:0007744|PDB:2EB3, ECO:0007744|PDB:2GS7, ECO:0007744|PDB:2ITN, ECO:0007744|PDB:2ITV, ECO:0007744|PDB:2ITX, ECO:0007744|PDB:3GT8, ECO:0007744|PDB:3VJN, ECO:0007744|PDB:3VJO, ECO:0007744|PDB:4RIW, ECO:0007744|PDB:4RIX, ECO:0007744|PDB:4RIY, ECO:0007744|PDB:4ZSE, ECO:0007744|PDB:5CNN, ECO:0007744|PDB:5CNO, ECO:0007744|PDB:5D41 |
| Chain | Residue | Details |
| A | THR766 | |
| B | THR766 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17349580, ECO:0000269|PubMed:19563760, ECO:0007744|PDB:2EB3, ECO:0007744|PDB:2GS7, ECO:0007744|PDB:2ITN, ECO:0007744|PDB:2ITV, ECO:0007744|PDB:2ITX, ECO:0007744|PDB:3GT8, ECO:0007744|PDB:3VJN, ECO:0007744|PDB:4RIW, ECO:0007744|PDB:4RIX, ECO:0007744|PDB:4RIY, ECO:0007744|PDB:4ZSE, ECO:0007744|PDB:5CNN, ECO:0007744|PDB:5CNO, ECO:0007744|PDB:5D41 |
| Chain | Residue | Details |
| A | ASP831 | |
| B | ASP831 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | SITE: Important for interaction with PIK3C2B |
| Chain | Residue | Details |
| A | TYR992 | |
| B | TYR992 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:21487020 |
| Chain | Residue | Details |
| A | SER205 | |
| B | SER205 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by PKC and PKD/PRKD1 => ECO:0000269|PubMed:10523301 |
| Chain | Residue | Details |
| A | THR654 | |
| B | THR654 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by PKD/PRKD1 => ECO:0000269|PubMed:10523301, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:3138233, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | THR669 | |
| B | THR669 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:3138233, ECO:0007744|PubMed:18691976 |
| Chain | Residue | Details |
| A | SER671 | |
| B | SER671 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-(2-hydroxyisobutyryl)lysine => ECO:0000269|PubMed:29192674 |
| Chain | Residue | Details |
| A | LYS721 | |
| B | LYS721 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:23774213 |
| Chain | Residue | Details |
| A | TYR845 | |
| B | TYR845 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:16083266, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER967 | |
| B | SER967 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 6 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | SER971 | |
| A | SER1015 | |
| A | SER1018 | |
| B | SER971 | |
| B | SER1015 | |
| B | SER1018 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:19563760, ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | TYR974 | |
| B | TYR974 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:19563760, ECO:0000269|PubMed:23774213 |
| Chain | Residue | Details |
| A | TYR992 | |
| B | TYR992 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:16083266, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER1002 | |
| B | SER1002 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648 |
| Chain | Residue | Details |
| A | THR1017 | |
| B | THR1017 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER1040 | |
| B | SER1040 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:23774213, ECO:0000305|PubMed:22888118 |
| Chain | Residue | Details |
| A | TYR1045 | |
| B | TYR1045 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:3138233 |
| Chain | Residue | Details |
| A | SER1046 | |
| A | SER1047 | |
| B | SER1046 | |
| B | SER1047 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18691976 |
| Chain | Residue | Details |
| A | SER1057 | |
| B | SER1057 |
| site_id | SWS_FT_FI26 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:12873986, ECO:0000269|PubMed:23774213, ECO:0000269|PubMed:27153536 |
| Chain | Residue | Details |
| A | TYR1068 | |
| B | TYR1068 |
| site_id | SWS_FT_FI27 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:12873986, ECO:0000269|PubMed:2543678 |
| Chain | Residue | Details |
| A | TYR1086 | |
| B | TYR1086 |
| site_id | SWS_FT_FI28 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER1142 | |
| B | SER1142 |
| site_id | SWS_FT_FI29 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:27153536, ECO:0007744|PubMed:17081983 |
| Chain | Residue | Details |
| A | TYR1148 | |
| B | TYR1148 |
| site_id | SWS_FT_FI30 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:19563760, ECO:0000269|PubMed:19836242, ECO:0000269|PubMed:23774213, ECO:0000269|PubMed:27153536, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | TYR1173 | |
| B | TYR1173 |
| site_id | SWS_FT_FI31 |
| Number of Residues | 2 |
| Details | MOD_RES: Omega-N-methylarginine => ECO:0000269|PubMed:21258366 |
| Chain | Residue | Details |
| A | ARG1175 | |
| B | ARG1175 |
| site_id | SWS_FT_FI32 |
| Number of Residues | 4 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:27153536 |
| Chain | Residue | Details |
| A | CYS1025 | |
| A | CYS1122 | |
| B | CYS1025 | |
| B | CYS1122 |
| site_id | SWS_FT_FI33 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) (complex) asparagine; atypical; partial => ECO:0000269|PubMed:10731668, ECO:0000269|PubMed:12297049, ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:1MOX, ECO:0007744|PDB:3NJP, ECO:0007744|PDB:3QWQ |
| Chain | Residue | Details |
| A | ASN32 | |
| B | ASN32 |
| site_id | SWS_FT_FI34 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine; atypical => ECO:0000269|PubMed:20837704, ECO:0007744|PDB:3NJP |
| Chain | Residue | Details |
| A | ASN49 | |
| B | ASN49 |
| site_id | SWS_FT_FI35 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:8962717 |
| Chain | Residue | Details |
| A | ASN104 | |
| B | ASN104 |
| site_id | SWS_FT_FI36 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0000269|PubMed:8962717, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:3NJP |
| Chain | Residue | Details |
| A | ASN151 | |
| B | ASN151 |
| site_id | SWS_FT_FI37 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12297049, ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:1MOX, ECO:0007744|PDB:3NJP |
| Chain | Residue | Details |
| A | ASN172 | |
| B | ASN172 |
| site_id | SWS_FT_FI38 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:10731668, ECO:0000269|PubMed:12297049, ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:20837704, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:1MOX, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9, ECO:0007744|PDB:3B2U, ECO:0007744|PDB:3B2V, ECO:0007744|PDB:3NJP, ECO:0007744|PDB:3P0Y, ECO:0007744|PDB:3QWQ, ECO:0007744|PDB:4KRL, ECO:0007744|PDB:4KRM, ECO:0007744|PDB:4KRO, ECO:0007744|PDB:4KRP, ECO:0007744|PDB:4UIP, ECO:0007744|PDB:4UV7 |
| Chain | Residue | Details |
| A | ASN328 | |
| B | ASN328 |
| site_id | SWS_FT_FI39 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:10731668, ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9, ECO:0007744|PDB:3B2U, ECO:0007744|PDB:3NJP, ECO:0007744|PDB:3QWQ, ECO:0007744|PDB:4KRL, ECO:0007744|PDB:4KRM, ECO:0007744|PDB:4KRO, ECO:0007744|PDB:4KRP, ECO:0007744|PDB:4UV7 |
| Chain | Residue | Details |
| A | ASN337 | |
| B | ASN337 |
| site_id | SWS_FT_FI40 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:8962717, ECO:0007744|PDB:1YY9, ECO:0007744|PDB:3B2U, ECO:0007744|PDB:3B2V, ECO:0007744|PDB:3P0Y, ECO:0007744|PDB:3QWQ, ECO:0007744|PDB:4KRO, ECO:0007744|PDB:4KRP |
| Chain | Residue | Details |
| A | ASN389 | |
| B | ASN389 |
| site_id | SWS_FT_FI41 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12297050, ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0000269|PubMed:8962717, ECO:0007744|PDB:1IVO, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9, ECO:0007744|PDB:3B2U, ECO:0007744|PDB:3B2V, ECO:0007744|PDB:3NJP, ECO:0007744|PDB:3P0Y, ECO:0007744|PDB:3QWQ, ECO:0007744|PDB:4KRL, ECO:0007744|PDB:4KRM, ECO:0007744|PDB:4KRO, ECO:0007744|PDB:4KRP, ECO:0007744|PDB:4UIP, ECO:0007744|PDB:4UV7 |
| Chain | Residue | Details |
| A | ASN420 | |
| B | ASN420 |
| site_id | SWS_FT_FI42 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:20837704, ECO:0000269|PubMed:25922362, ECO:0000269|PubMed:8962717, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9, ECO:0007744|PDB:3B2V, ECO:0007744|PDB:3NJP, ECO:0007744|PDB:3QWQ, ECO:0007744|PDB:4KRP, ECO:0007744|PDB:4UIP, ECO:0007744|PDB:4UV7 |
| Chain | Residue | Details |
| A | ASN504 | |
| B | ASN504 |
| site_id | SWS_FT_FI43 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine; partial => ECO:0000269|PubMed:10731668, ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0000269|PubMed:19159218, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9 |
| Chain | Residue | Details |
| A | ASN544 | |
| B | ASN544 |
| site_id | SWS_FT_FI44 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine; partial => ECO:0000269|PubMed:10731668, ECO:0000269|PubMed:12620237, ECO:0000269|PubMed:12731890, ECO:0000269|PubMed:16083266, ECO:0007744|PDB:1NQL, ECO:0007744|PDB:1YY9 |
| Chain | Residue | Details |
| A | ASN579 | |
| B | ASN579 |
| site_id | SWS_FT_FI45 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) (high mannose) asparagine => ECO:0000305|PubMed:12731890 |
| Chain | Residue | Details |
| A | ASN599 | |
| B | ASN599 |
| site_id | SWS_FT_FI46 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:33996800 |
| Chain | Residue | Details |
| A | LYS692 | |
| A | LYS713 | |
| A | LYS730 | |
| A | LYS843 | |
| B | LYS692 | |
| B | LYS713 | |
| B | LYS730 | |
| B | LYS843 |
| site_id | SWS_FT_FI47 |
| Number of Residues | 6 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144 |
| Chain | Residue | Details |
| A | LYS905 | |
| A | LYS946 | |
| B | LYS905 | |
| B | LYS946 |
| site_id | SWS_FT_FI48 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:33996800 |
| Chain | Residue | Details |
| A | LYS733 | |
| A | LYS936 | |
| B | LYS733 | |
| B | LYS936 |
