7QNC
Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004888 | molecular_function | transmembrane signaling receptor activity |
| A | 0004890 | molecular_function | GABA-A receptor activity |
| A | 0005216 | molecular_function | monoatomic ion channel activity |
| A | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| A | 0006811 | biological_process | monoatomic ion transport |
| A | 0006821 | biological_process | chloride transport |
| A | 0007214 | biological_process | gamma-aminobutyric acid signaling pathway |
| A | 0016020 | cellular_component | membrane |
| A | 0034220 | biological_process | monoatomic ion transmembrane transport |
| B | 0004888 | molecular_function | transmembrane signaling receptor activity |
| B | 0004890 | molecular_function | GABA-A receptor activity |
| B | 0005216 | molecular_function | monoatomic ion channel activity |
| B | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0016020 | cellular_component | membrane |
| B | 0034220 | biological_process | monoatomic ion transmembrane transport |
| C | 0004888 | molecular_function | transmembrane signaling receptor activity |
| C | 0004890 | molecular_function | GABA-A receptor activity |
| C | 0005216 | molecular_function | monoatomic ion channel activity |
| C | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| C | 0006811 | biological_process | monoatomic ion transport |
| C | 0016020 | cellular_component | membrane |
| C | 0034220 | biological_process | monoatomic ion transmembrane transport |
| D | 0004888 | molecular_function | transmembrane signaling receptor activity |
| D | 0004890 | molecular_function | GABA-A receptor activity |
| D | 0005216 | molecular_function | monoatomic ion channel activity |
| D | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| D | 0006811 | biological_process | monoatomic ion transport |
| D | 0016020 | cellular_component | membrane |
| D | 0034220 | biological_process | monoatomic ion transmembrane transport |
| E | 0004888 | molecular_function | transmembrane signaling receptor activity |
| E | 0004890 | molecular_function | GABA-A receptor activity |
| E | 0005216 | molecular_function | monoatomic ion channel activity |
| E | 0005230 | molecular_function | extracellular ligand-gated monoatomic ion channel activity |
| E | 0006811 | biological_process | monoatomic ion transport |
| E | 0016020 | cellular_component | membrane |
| E | 0034220 | biological_process | monoatomic ion transmembrane transport |
Functional Information from PROSITE/UniProt
| site_id | PS00236 |
| Number of Residues | 15 |
| Details | NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CpMrLvdFPmDghaC |
| Chain | Residue | Details |
| A | CYS172-CYS186 | |
| E | CYS164-CYS178 | |
| B | CYS136-CYS150 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 237 |
| Details | TOPO_DOM: Extracellular => ECO:0000305 |
| Chain | Residue | Details |
| E | MET25-ILE251 | |
| E | SER299-LYS308 | |
| E | TYR450-MET452 | |
| C | LEU268-LYS279 | |
| D | GLN1-PHE221 | |
| D | LEU268-LYS279 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 85 |
| Details | TRANSMEM: Helical => ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| E | ILE252-ILE271 | |
| D | SER247-HIS267 | |
| D | ALA280-ASN303 | |
| D | ILE423-TYR445 | |
| E | VAL276-ARG298 | |
| E | ALA309-ALA331 | |
| E | ILE427-ALA449 | |
| C | ILE222-ILE242 | |
| C | SER247-HIS267 | |
| C | ALA280-ASN303 | |
| C | ILE423-TYR445 | |
| D | ILE222-ILE242 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 97 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000305 |
| Chain | Residue | Details |
| E | SER272-ALA275 | |
| E | HIS332-THR426 | |
| C | ASN243-ALA246 | |
| C | TYR304-ALA422 | |
| D | ASN243-ALA246 | |
| D | TYR304-ALA422 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P18506 |
| Chain | Residue | Details |
| E | SER390 | |
| C | TYR446-ASN448 | |
| D | TYR446-ASN448 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| E | ASN103 | |
| B | PHE200 | |
| C | ASP95 | |
| C | PHE200 | |
| D | ASP95 | |
| D | PHE200 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| E | ASN106 | |
| B | SER156 | |
| B | THR202 | |
| C | TYR97 | |
| C | SER156 | |
| C | THR202 | |
| D | TYR97 | |
| D | SER156 | |
| D | THR202 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | BINDING: in chain A => ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| B | GLU155 | |
| C | GLU155 | |
| D | GLU155 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 3 |
| Details | BINDING: in chain A => ECO:0000269|PubMed:30140029, ECO:0007744|PDB:6A96 |
| Chain | Residue | Details |
| B | TYR157 | |
| C | TYR157 | |
| D | TYR157 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| B | ASN8 | |
| C | ASN8 | |
| D | ASN8 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:30602789, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:6I53, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| B | ASN80 | |
| C | ASN80 | |
| D | ASN80 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:30140029, ECO:0000269|PubMed:30602789, ECO:0000269|PubMed:35355020, ECO:0007744|PDB:6A96, ECO:0007744|PDB:6I53, ECO:0007744|PDB:7QN7 |
| Chain | Residue | Details |
| B | ASN149 | |
| C | ASN149 | |
| D | ASN149 |
