7QNC
Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Gamma-aminobutyric acid receptor subunit alpha-4 | polymer | 554 | 61696.0 | 1 | UniProt (P48169) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-4 |
| 2 | B, C, D (B, C, D) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54180.3 | 3 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 3 | E (E) | Gamma-aminobutyric acid receptor subunit delta | polymer | 472 | 52437.9 | 1 | UniProt (O14764) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit delta |
| 4 | F, G (F, G) | Nanobody Nb25 | polymer | 121 | 13341.7 | 2 | Lama glama | ||
| 5 | H, J, L, N (a, H, I, J) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 4 | In PDB GlyTouCan (G22768VO) | |||
| 6 | I, K, M (b, c, d) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | AA, O, Q, T, W... (E, A, B, C, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
| 8 | P, S, V (B, C, D) | DECANE | non-polymer | 142.3 | 3 | Chemie (D10) | |||
| 9 | R, Z (B, E) | 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one | non-polymer | 140.1 | 2 | Chemie (EI7) | |||
| 10 | U, X (C, D) | HISTAMINE | non-polymer | 111.1 | 2 | Chemie (HSM) |
Sequence modifications
E: 1 - 452 (UniProt: O14764)
| PDB | External Database | Details |
|---|---|---|
| Gly 453 | - | expression tag |
| Gly 454 | - | expression tag |
| Ser 455 | - | expression tag |
| Gly 456 | - | expression tag |
| Gly 457 | - | expression tag |
| Ser 458 | - | expression tag |
| Gly 459 | - | expression tag |
| Gly 460 | - | expression tag |
| Ser 461 | - | expression tag |
| Gly 462 | - | expression tag |
| Lys 463 | - | expression tag |
| Thr 464 | - | expression tag |
| Glu 465 | - | expression tag |
| Thr 466 | - | expression tag |
| Ser 467 | - | expression tag |
| Gln 468 | - | expression tag |
| Val 469 | - | expression tag |
| Ala 470 | - | expression tag |
| Pro 471 | - | expression tag |
| Ala 472 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 303358.5 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 4916.5 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2256.7 | |
| All* | Total formula weight | 310531.7 |
