7B5R

Ubiquitin ligation to F-box protein substrates by SCF-RBR E3-E3 super-assembly: CUL1-RBX1-SKP1-SKP2-CKSHS1-Cyclin A-CDK2-p27

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0006513	biological_process	protein monoubiquitination
C	0006915	biological_process	apoptotic process
C	0008283	biological_process	cell population proliferation
C	0009887	biological_process	animal organ morphogenesis
C	0016567	biological_process	protein ubiquitination
C	0019005	cellular_component	SCF ubiquitin ligase complex
C	0030674	molecular_function	protein-macromolecule adaptor activity
C	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0034599	biological_process	cellular response to oxidative stress
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0070936	biological_process	protein K48-linked ubiquitination
C	0097193	biological_process	intrinsic apoptotic signaling pathway
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	1990452	cellular_component	Parkin-FBXW7-Cul1 ubiquitin ligase complex
K	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
K	0005515	molecular_function	protein binding
K	0005654	cellular_component	nucleoplasm
K	0007346	biological_process	regulation of mitotic cell cycle
K	0016538	molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity
K	0019005	cellular_component	SCF ubiquitin ligase complex
K	0019901	molecular_function	protein kinase binding
K	0042393	molecular_function	histone binding
K	0043130	molecular_function	ubiquitin binding
K	0051301	biological_process	cell division
K	0061575	molecular_function	cyclin-dependent protein serine/threonine kinase activator activity
L	0000082	biological_process	G1/S transition of mitotic cell cycle
L	0000086	biological_process	G2/M transition of mitotic cell cycle
L	0000122	biological_process	negative regulation of transcription by RNA polymerase II
L	0000166	molecular_function	nucleotide binding
L	0000287	molecular_function	magnesium ion binding
L	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
L	0000781	cellular_component	chromosome, telomeric region
L	0000793	cellular_component	condensed chromosome
L	0000805	cellular_component	X chromosome
L	0000806	cellular_component	Y chromosome
L	0001673	cellular_component	male germ cell nucleus
L	0004672	molecular_function	protein kinase activity
L	0004674	molecular_function	protein serine/threonine kinase activity
L	0004693	molecular_function	cyclin-dependent protein serine/threonine kinase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0005635	cellular_component	nuclear envelope
L	0005654	cellular_component	nucleoplasm
L	0005667	cellular_component	transcription regulator complex
L	0005737	cellular_component	cytoplasm
L	0005768	cellular_component	endosome
L	0005813	cellular_component	centrosome
L	0005829	cellular_component	cytosol
L	0006260	biological_process	DNA replication
L	0006281	biological_process	DNA repair
L	0006338	biological_process	chromatin remodeling
L	0006351	biological_process	DNA-templated transcription
L	0006468	biological_process	protein phosphorylation
L	0006813	biological_process	potassium ion transport
L	0006974	biological_process	DNA damage response
L	0007099	biological_process	centriole replication
L	0007165	biological_process	signal transduction
L	0007265	biological_process	Ras protein signal transduction
L	0007346	biological_process	regulation of mitotic cell cycle
L	0008284	biological_process	positive regulation of cell population proliferation
L	0010389	biological_process	regulation of G2/M transition of mitotic cell cycle
L	0010468	biological_process	regulation of gene expression
L	0015030	cellular_component	Cajal body
L	0016301	molecular_function	kinase activity
L	0016740	molecular_function	transferase activity
L	0018105	biological_process	peptidyl-serine phosphorylation
L	0019904	molecular_function	protein domain specific binding
L	0030332	molecular_function	cyclin binding
L	0031453	biological_process	positive regulation of heterochromatin formation
L	0031571	biological_process	mitotic G1 DNA damage checkpoint signaling
L	0032298	biological_process	positive regulation of DNA-templated DNA replication initiation
L	0035173	molecular_function	histone kinase activity
L	0043247	biological_process	telomere maintenance in response to DNA damage
L	0043687	biological_process	post-translational protein modification
L	0045740	biological_process	positive regulation of DNA replication
L	0045893	biological_process	positive regulation of DNA-templated transcription
L	0046872	molecular_function	metal ion binding
L	0051298	biological_process	centrosome duplication
L	0051301	biological_process	cell division
L	0051321	biological_process	meiotic cell cycle
L	0071732	biological_process	cellular response to nitric oxide
L	0090398	biological_process	cellular senescence
L	0097123	cellular_component	cyclin A1-CDK2 complex
L	0097124	cellular_component	cyclin A2-CDK2 complex
L	0097134	cellular_component	cyclin E1-CDK2 complex
L	0097135	cellular_component	cyclin E2-CDK2 complex
L	0097472	molecular_function	cyclin-dependent protein kinase activity
L	0106310	molecular_function	protein serine kinase activity
L	0120186	biological_process	negative regulation of protein localization to chromatin
L	0120261	biological_process	regulation of heterochromatin organization
L	1905784	biological_process	regulation of anaphase-promoting complex-dependent catabolic process
P	0000079	biological_process	regulation of cyclin-dependent protein serine/threonine kinase activity
P	0000082	biological_process	G1/S transition of mitotic cell cycle
P	0001890	biological_process	placenta development
P	0004860	molecular_function	protein kinase inhibitor activity
P	0004861	molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005768	cellular_component	endosome
P	0005813	cellular_component	centrosome
P	0005829	cellular_component	cytosol
P	0005929	cellular_component	cilium
P	0006813	biological_process	potassium ion transport
P	0006915	biological_process	apoptotic process
P	0007096	biological_process	regulation of exit from mitosis
P	0007165	biological_process	signal transduction
P	0007219	biological_process	Notch signaling pathway
P	0007346	biological_process	regulation of mitotic cell cycle
P	0007507	biological_process	heart development
P	0007605	biological_process	sensory perception of sound
P	0008284	biological_process	positive regulation of cell population proliferation
P	0008285	biological_process	negative regulation of cell population proliferation
P	0012501	biological_process	programmed cell death
P	0019901	molecular_function	protein kinase binding
P	0019903	molecular_function	protein phosphatase binding
P	0019914	molecular_function	cyclin-dependent protein kinase regulator activity
P	0030308	biological_process	negative regulation of cell growth
P	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
P	0030332	molecular_function	cyclin binding
P	0030334	biological_process	regulation of cell migration
P	0031116	biological_process	positive regulation of microtubule polymerization
P	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
P	0031625	molecular_function	ubiquitin protein ligase binding
P	0035869	cellular_component	ciliary transition zone
P	0036064	cellular_component	ciliary basal body
P	0044877	molecular_function	protein-containing complex binding
P	0045732	biological_process	positive regulation of protein catabolic process
P	0045740	biological_process	positive regulation of DNA replication
P	0045926	biological_process	negative regulation of growth
P	0045930	biological_process	negative regulation of mitotic cell cycle
P	0048102	biological_process	autophagic cell death
P	0048839	biological_process	inner ear development
P	0050680	biological_process	negative regulation of epithelial cell proliferation
P	0051087	molecular_function	protein-folding chaperone binding
P	0051168	biological_process	nuclear export
P	0051726	biological_process	regulation of cell cycle
P	0060090	molecular_function	molecular adaptor activity
P	0060767	biological_process	epithelial cell proliferation involved in prostate gland development
P	0060770	biological_process	negative regulation of epithelial cell proliferation involved in prostate gland development
P	0071236	biological_process	cellular response to antibiotic
P	0071285	biological_process	cellular response to lithium ion
P	0090398	biological_process	cellular senescence
P	0140678	molecular_function	molecular function inhibitor activity
P	1901990	biological_process	regulation of mitotic cell cycle phase transition
P	1902746	biological_process	regulation of lens fiber cell differentiation
P	1902806	biological_process	regulation of cell cycle G1/S phase transition
P	1904019	biological_process	epithelial cell apoptotic process
P	1904036	biological_process	negative regulation of epithelial cell apoptotic process
P	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
P	1905179	biological_process	negative regulation of cardiac muscle tissue regeneration
P	1990757	molecular_function	ubiquitin ligase activator activity
P	2000045	biological_process	regulation of G1/S transition of mitotic cell cycle
S	0000209	biological_process	protein polyubiquitination
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005829	cellular_component	cytosol
S	0006338	biological_process	chromatin remodeling
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0006513	biological_process	protein monoubiquitination
S	0008013	molecular_function	beta-catenin binding
S	0016567	biological_process	protein ubiquitination
S	0019005	cellular_component	SCF ubiquitin ligase complex
S	0019904	molecular_function	protein domain specific binding
S	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
S	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
S	0031519	cellular_component	PcG protein complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051457	biological_process	maintenance of protein location in nucleus
S	0070936	biological_process	protein K48-linked ubiquitination
S	0097602	molecular_function	cullin family protein binding
S	0140677	molecular_function	molecular function activator activity
S	0160072	molecular_function	ubiquitin ligase complex scaffold activity
S	1990444	molecular_function	F-box domain binding
S	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
S	1990757	molecular_function	ubiquitin ligase activator activity
T	0000082	biological_process	G1/S transition of mitotic cell cycle
T	0000086	biological_process	G2/M transition of mitotic cell cycle
T	0000209	biological_process	protein polyubiquitination
T	0002376	biological_process	immune system process
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005730	cellular_component	nucleolus
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0016567	biological_process	protein ubiquitination
T	0019005	cellular_component	SCF ubiquitin ligase complex
T	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
T	0033148	biological_process	positive regulation of intracellular estrogen receptor signaling pathway
T	0042802	molecular_function	identical protein binding
T	0042981	biological_process	regulation of apoptotic process
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0045087	biological_process	innate immune response
T	0048661	biological_process	positive regulation of smooth muscle cell proliferation
T	0051607	biological_process	defense response to virus
T	0051726	biological_process	regulation of cell cycle
T	0070534	biological_process	protein K63-linked ubiquitination
T	0070936	biological_process	protein K48-linked ubiquitination
T	0071460	biological_process	cellular response to cell-matrix adhesion
T	1902916	biological_process	positive regulation of protein polyubiquitination
T	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
T	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
Y	0000082	biological_process	G1/S transition of mitotic cell cycle
Y	0000086	biological_process	G2/M transition of mitotic cell cycle
Y	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
Y	0001939	cellular_component	female pronucleus
Y	0001940	cellular_component	male pronucleus
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005815	cellular_component	microtubule organizing center
Y	0005829	cellular_component	cytosol
Y	0006275	biological_process	regulation of DNA replication
Y	0006351	biological_process	DNA-templated transcription
Y	0007265	biological_process	Ras protein signal transduction
Y	0016538	molecular_function	cyclin-dependent protein serine/threonine kinase regulator activity
Y	0019901	molecular_function	protein kinase binding
Y	0019904	molecular_function	protein domain specific binding
Y	0031100	biological_process	animal organ regeneration
Y	0032355	biological_process	response to estradiol
Y	0033762	biological_process	response to glucagon
Y	0036120	biological_process	cellular response to platelet-derived growth factor stimulus
Y	0043687	biological_process	post-translational protein modification
Y	0044320	biological_process	cellular response to leptin stimulus
Y	0044772	biological_process	mitotic cell cycle phase transition
Y	0044843	biological_process	cell cycle G1/S phase transition
Y	0045893	biological_process	positive regulation of DNA-templated transcription
Y	0048146	biological_process	positive regulation of fibroblast proliferation
Y	0051301	biological_process	cell division
Y	0071314	biological_process	cellular response to cocaine
Y	0071373	biological_process	cellular response to luteinizing hormone stimulus
Y	0071392	biological_process	cellular response to estradiol stimulus
Y	0071456	biological_process	cellular response to hypoxia
Y	0071732	biological_process	cellular response to nitric oxide
Y	0090102	biological_process	cochlea development
Y	0097122	cellular_component	cyclin A2-CDK1 complex
Y	0097124	cellular_component	cyclin A2-CDK2 complex
Y	1990314	biological_process	cellular response to insulin-like growth factor stimulus
Y	2000573	biological_process	positive regulation of DNA biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGEGTYGVVYkArnkltgev..........VALK

Chain	Residue	Details
L	ILE10-LYS33

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VlHrDLKpqNLLI

Chain	Residue	Details
L	VAL123-ILE135

---

site_id	PS00292
Number of Residues	32
Details	CYCLINS Cyclins signature. RaiLvdWLvevgeeykLqnetLhlAVnYIDRF

Chain	Residue	Details
Y	ARG211-PHE242

---

site_id	PS00944
Number of Residues	19
Details	CKS_1 Cyclin-dependent kinases regulatory subunits signature 1. YSdKYdDEeFEYRHVmLPK

Chain	Residue	Details
K	TYR8-LYS26

---

site_id	PS00945
Number of Residues	11
Details	CKS_2 Cyclin-dependent kinases regulatory subunits signature 2. HePEpHILLFR

Chain	Residue	Details
K	HIS60-ARG70

---

site_id	PS01256
Number of Residues	28
Details	CULLIN_1 Cullin family signature. IKkcIdiLIEKeYLeRvdgekdtYsYlA

Chain	Residue	Details
C	ILE749-ALA776

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	25
Details	Repeat: {"description":"LRR 1","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	27
Details	Repeat: {"description":"LRR 2","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Repeat: {"description":"LRR 3","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	22
Details	Repeat: {"description":"LRR 4","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	26
Details	Repeat: {"description":"LRR 5","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	22
Details	Repeat: {"description":"LRR 6","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	21
Details	Repeat: {"description":"LRR 7","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	22
Details	Repeat: {"description":"LRR 8","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	19
Details	Repeat: {"description":"LRR 9","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	21
Details	Repeat: {"description":"LRR 10","evidences":[{"source":"PubMed","id":"11099048","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18220336","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)","evidences":[{"source":"PubMed","id":"25114211","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17095507","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21565702","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21565702","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Site: {"description":"CDK7 binding","evidences":[{"source":"PubMed","id":"17373709","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"1396589","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17095507","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by WEE1","evidences":[{"source":"PubMed","id":"1396589","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17095507","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by CAK and CCRK","evidences":[{"source":"PubMed","id":"1396589","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"14597612","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16325401","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17095507","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17570665","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20147522","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20360007","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21565702","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28666995","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	40
Details	Region: {"description":"Interaction with CDK2","evidences":[{"source":"PubMed","id":"28666995","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by SRC","evidences":[{"source":"PubMed","id":"17254967","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine; by ABL, LYN, SRC and JAK2","evidences":[{"source":"PubMed","id":"16195327","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17254966","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17254967","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21423214","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"16195327","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine; by PKB/AKT1, CDK1 and CDK2","evidences":[{"source":"PubMed","id":"10323868","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"12042314","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16209941","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"23478441","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---