Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VZQ

Engineered TTLL6 mutant bound to alpha-elongation analog

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0036211	biological_process	protein modification process
B	0036211	biological_process	protein modification process
C	0036211	biological_process	protein modification process
D	0036211	biological_process	protein modification process

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	binding site for residue ADP A 601

Chain	Residue
A	LYS125
A	LEU203
A	TYR204
A	ILE205
A	LYS215
A	ASP217
A	HIS261
A	THR263
A	ASN264
A	ASP346
A	LEU348
A	PRO147
A	LEU358
A	GLU359
A	RZY602
A	MG603
A	MG604
A	ILE172
A	LYS174
A	ALA179
A	ARG180
A	GLY181
A	ILE184
A	GLN202

site_id	AC2
Number of Residues	22
Details	binding site for residue RZY A 602

Chain	Residue
A	TYR69
A	ALA179
A	ARG180
A	ARG219
A	TYR221
A	LEU239
A	ARG241
A	ASN264
A	TYR265
A	SER266
A	LYS283
A	ASP346
A	GLU359
A	ASN361
A	SER363
A	PRO364
A	SER365
A	ASP373
A	LYS377
A	ADP601
A	MG603
A	MG604

site_id	AC3
Number of Residues	7
Details	binding site for residue MG A 603

Chain	Residue
A	ARG241
A	ASN264
A	ASP346
A	GLU359
A	ADP601
A	RZY602
A	MG604

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 604

Chain	Residue
A	LYS125
A	GLU359
A	ASN361
A	ADP601
A	RZY602
A	MG603

site_id	AC5
Number of Residues	23
Details	binding site for residue ADP B 601

Chain	Residue
B	LYS125
B	ILE172
B	LYS174
B	ALA179
B	ARG180
B	GLY181
B	ILE184
B	GLN202
B	LEU203
B	TYR204
B	ILE205
B	LYS215
B	ASP217
B	LEU262
B	THR263
B	ASN264
B	ASP346
B	LEU348
B	LEU358
B	GLU359
B	RZP604
B	MG605
B	MG606

site_id	AC6
Number of Residues	5
Details	binding site for residue GOL B 602

Chain	Residue
B	ARG68
B	TYR69
B	ASP70
B	SER367
B	THR368

site_id	AC7
Number of Residues	3
Details	binding site for residue GOL B 603

Chain	Residue
B	LYS169
B	LEU203
B	GOL608

site_id	AC8
Number of Residues	22
Details	binding site for residue RZP B 604

Chain	Residue
B	LYS283
B	ASP346
B	GLU359
B	ASN361
B	SER363
B	PRO364
B	SER365
B	LYS377
B	ADP601
B	MG605
B	MG606
B	GOL607
D	LYS194
B	TYR69
B	ALA179
B	ARG180
B	ARG219
B	LEU239
B	ARG241
B	ASN264
B	TYR265
B	SER266

site_id	AC9
Number of Residues	6
Details	binding site for residue MG B 605

Chain	Residue
B	ARG241
B	ASN264
B	ASP346
B	GLU359
B	ADP601
B	RZP604

site_id	AD1
Number of Residues	4
Details	binding site for residue MG B 606

Chain	Residue
B	GLU359
B	ASN361
B	ADP601
B	RZP604

site_id	AD2
Number of Residues	4
Details	binding site for residue GOL B 607

Chain	Residue
B	ARG68
B	SER363
B	RZP604
D	GLU192

site_id	AD3
Number of Residues	4
Details	binding site for residue GOL B 608

Chain	Residue
B	HIS144
B	PHE146
B	ARG148
B	GOL603

site_id	AD4
Number of Residues	6
Details	binding site for residue GOL B 609

Chain	Residue
B	TYR235
B	ASN236
B	ASN290
B	TRP304
B	ARG445
B	GOL610

site_id	AD5
Number of Residues	5
Details	binding site for residue GOL B 610

Chain	Residue
B	LYS294
B	GLU301
B	TRP304
B	ARG305
B	GOL609

site_id	AD6
Number of Residues	9
Details	binding site for residue GOL B 611

Chain	Residue
B	PHE214
B	PHE242
B	ALA243
B	THR244
B	ASN273
B	SER280
B	GLY281
B	SER282
B	ARG284

site_id	AD7
Number of Residues	23
Details	binding site for residue ADP C 601

Chain	Residue
C	LYS125
C	ILE172
C	LYS174
C	ALA179
C	ARG180
C	GLY181
C	ILE184
C	GLN202
C	LEU203
C	TYR204
C	ILE205
C	LYS215
C	ASP217
C	HIS261
C	THR263
C	ASN264
C	ASP346
C	LEU348
C	LEU358
C	GLU359
C	RZP602
C	MG603
C	MG604

site_id	AD8
Number of Residues	23
Details	binding site for residue RZP C 602

Chain	Residue
A	LYS194
C	TYR69
C	GLY178
C	ALA179
C	ARG180
C	ARG219
C	TYR221
C	ARG241
C	ASN264
C	TYR265
C	SER266
C	LYS283
C	ASP346
C	GLU359
C	ASN361
C	ILE362
C	SER363
C	PRO364
C	SER365
C	LYS377
C	ADP601
C	MG603
C	MG604

site_id	AD9
Number of Residues	8
Details	binding site for residue MG C 603

Chain	Residue
C	LYS125
C	GLY178
C	ALA179
C	ARG180
C	GLU359
C	ASN361
C	ADP601
C	RZP602

site_id	AE1
Number of Residues	6
Details	binding site for residue MG C 604

Chain	Residue
C	ARG241
C	ASN264
C	ASP346
C	GLU359
C	ADP601
C	RZP602

site_id	AE2
Number of Residues	1
Details	binding site for residue GOL C 605

Chain	Residue
C	LEU203

site_id	AE3
Number of Residues	25
Details	binding site for residue ADP D 601

Chain	Residue
D	LYS125
D	ILE172
D	LYS174
D	ALA179
D	ARG180
D	GLY181
D	ILE184
D	GLN202
D	LEU203
D	TYR204
D	ILE205
D	LYS215
D	ASP217
D	HIS261
D	LEU262
D	THR263
D	ASN264
D	ASP346
D	LEU348
D	LEU358
D	GLU359
D	RZP602
D	MG603
D	MG604
D	HOH701

site_id	AE4
Number of Residues	26
Details	binding site for residue RZP D 602

Chain	Residue
B	LYS194
D	TYR69
D	ARG180
D	ARG219
D	TYR221
D	LEU239
D	ARG241
D	ASN264
D	TYR265
D	SER266
D	LYS283
D	ASP346
D	GLU359
D	ASN361
D	ILE362
D	SER363
D	PRO364
D	SER365
D	LYS377
D	ADP601
D	MG603
D	MG604
D	GOL611
D	HOH701
D	HOH702
D	HOH704

site_id	AE5
Number of Residues	7
Details	binding site for residue MG D 603

Chain	Residue
D	ASP346
D	GLU359
D	ASN361
D	ADP601
D	RZP602
D	MG604
D	HOH701

site_id	AE6
Number of Residues	5
Details	binding site for residue MG D 604

Chain	Residue
D	GLU359
D	ASN361
D	ADP601
D	RZP602
D	MG603

site_id	AE7
Number of Residues	2
Details	binding site for residue GOL D 605

Chain	Residue
D	LYS294
D	TYR298

site_id	AE8
Number of Residues	2
Details	binding site for residue GOL D 606

Chain	Residue
D	ASP309
D	LYS455

site_id	AE9
Number of Residues	7
Details	binding site for residue GOL D 607

Chain	Residue
D	PHE214
D	PHE242
D	ALA243
D	SER280
D	GLY281
D	SER282
D	ARG284

site_id	AF1
Number of Residues	4
Details	binding site for residue GOL D 608

Chain	Residue
D	ASP212
D	TYR292
D	HIS296
D	GOL609

site_id	AF2
Number of Residues	5
Details	binding site for residue GOL D 609

Chain	Residue
D	ILE210
D	ASP212
D	GLY213
D	GOL608
D	GOL613

site_id	AF3
Number of Residues	4
Details	binding site for residue GOL D 610

Chain	Residue
D	GLU301
D	TRP304
D	ARG305
D	ARG445

site_id	AF4
Number of Residues	4
Details	binding site for residue GOL D 611

Chain	Residue
B	LYS194
D	ARG68
D	TYR97
D	RZP602

site_id	AF5
Number of Residues	3
Details	binding site for residue GOL D 612

Chain	Residue
D	LEU203
D	ILE205
D	GOL614

site_id	AF6
Number of Residues	4
Details	binding site for residue GOL D 613

Chain	Residue
D	PHE209
D	ILE210
D	ARG351
D	GOL609

site_id	AF7
Number of Residues	4
Details	binding site for residue GOL D 614

Chain	Residue
D	HIS144
D	ARG148
D	TRP356
D	GOL612

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZT

Chain	Residue	Details
A	LYS174
C	GLN202
C	LYS215
C	THR263
D	LYS174
D	GLN202
D	LYS215
D	THR263
A	GLN202
A	LYS215
A	THR263
B	LYS174
B	GLN202
B	LYS215
B	THR263
C	LYS174

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782

Chain	Residue	Details
A	ARG180
A	ILE362
B	ARG180
B	ILE362
C	ARG180
C	ILE362
D	ARG180
D	ILE362

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782, ECO:0007744\|PDB:6VZU, ECO:0007744\|PDB:6VZW

Chain	Residue	Details
A	ARG241
B	LYS377
C	ARG241
C	TYR265
C	SER266
C	LYS283
C	LYS377
D	ARG241
D	TYR265
D	SER266
D	LYS283
A	TYR265
D	LYS377
A	SER266
A	LYS283
A	LYS377
B	ARG241
B	TYR265
B	SER266
B	LYS283

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZU

Chain	Residue	Details
A	ASP346
D	ASP346
D	GLU359
D	ASN361
A	GLU359
A	ASN361
B	ASP346
B	GLU359
B	ASN361
C	ASP346
C	GLU359
C	ASN361

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Essential for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ARG180
B	ARG180
C	ARG180
D	ARG180

site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Important for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ILE362
B	ILE362
C	ILE362
D	ILE362

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)