6VZQ

Engineered TTLL6 mutant bound to alpha-elongation analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	75.038, 109.491, 171.700
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	46.160 - 3.080
R-factor	0.2277
Rwork	0.226
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylr
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	3.194
High resolution limit [Å]	3.080	3.080
Rmerge	0.061	0.251
Rmeas	0.086	0.355
Rpim	0.060	0.251
Number of reflections	101188	9901
<I/σ(I)>	7.16	2.65
Completeness [%]	99.6	98.36
Redundancy	2	2
CC(1/2)	0.995	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	100 mM Sodium Citrate, pH 6.2, 200 mM MgCl2, 12% Peg 20000